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- 2018-06-28 发布于上海
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采用微生物发酵制取丙酮酸的分析-analysis of pyruvic acid production by microbial fermentation
硕士论文采埘微生物发酵制取丙酮酸的研究y 624849摘要采用微生物发酵技术制取丙酮酸,利用物理的和化学的方法进行诱变,获得 一株汉逊德巴利酵母汉逊变种N039一I。通过优化培养条件,该突变菌株生产丙 酮酸的能力较出发菌株提高了1.8倍。在30。C,200r/min摇瓶培养72h的条件下, 发酵液中丙酮酸含量达到5.29/L。关键词:酵母,发酵,丙酮酸,诱变硕士论文采川微生物发酵制取丙酮酸的研究AbstractFermentative production of pyruvic acid by Debaryomyces hansii vat.hansenii was investigated in this paper.A mutant was obtained after DES and UV treatment.By using orthogonal experiment method,the concentration of pyruvic acid reached 5.29/L at 30。C,200r/min,72h in flask culture.Key word:yeast,fermentation,pyruvic acid,mutantionIf堡主望奎。墨型壁兰笪垄些型坚娈塑塑堕翌塞1引言1.1立题依据丙酮酸(Pyruvic acid),分子式为CH:jCOCO
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