吡格列酮对2型糖尿病大鼠肾脏及nf-κb mcp-1表达影响-effect of pioglitazone on expression of nf - κ b mcp - 1 in kidney of type 2 diabetic rats.docxVIP

TCandTGlevelingroupsDMandgroupPIOincreasedsignificantlycomparedwiththoseingroupNC(P0.01).TreatmentwithpioglitazonesignificantlyreducedtheTCandTG(P0.01).NF-κB,SMCP-1level,UMCP-1excretionrate,ACR,URBPexcretionrateandkidney/bodyweightratioingroupsDMandgroupPIOincreasedsignificantlycomparedwiththoseingroupNC(P0.01).TreatmentwithpioglitazonealsosignificantlyreducedNF-κB,SMCP-1level,UMCP-1excretionrate,ACR,URBPexcretionrateaswellaskidney/bodyweightratio(P0.01).Inaddition,UMCP-1excretionrateshowedpositivecorrelationswithACR,URBPexcretionrateandkidney/bodyweightratioandNF-κB,SMCP-1levelalsoshowedpositivecorrelationswith,URBPexcretionrateandkidney/bodyweightratio.TheexpressionlevelofMCP-1,NF-κBproteininglomcrulusandnephrictubuleingroupDMandgroupPIOwassignificantlyhigher(P0.01)thanthatingroupNC,pioglitazonecaninhibittheexpressionofMCP-1、NF-κBprotein(P0.01).Thelightmicroscopesresultsshowedthatthere’snopathologicallesionofkidneyfoundingroupNC.PathologicalchangesweremuchmoreobviousingroupDM.Itwasclearthattheglomerularcapillaryloopsweretumbling,lumensblocked,mesangialregionwidened,basallaminathickened,mesenteriumbaseincreased,thevolumeofglomerulusbecamelargerandthecellpopulationincreasedsignificantly.Italsocanbeobservedthattherenaltubulewasvacuolizationandtherenalinterstitiumwasinfiltratedbylotsoflymphocytesandemononuclearmacrophages.PathologicalchangesingroupPIOwerelighter,itcanbeobservedthatglomerularcapillarylumenwasconstrictiveslightlyandfewlymphocyteinfiltrated.Theelectronmicroscopesresultsshowedthatthestructureandwidthofglomerularbasementmembrane(GBM),epithelialfootprocesses(FP)aswellasthemesangialregionwerenormalingroupNCatthe8thweek.IngroupDM,theFPfusionratewasapproximately80percentandthethickeningofGBMwereobserved.itwasnotedthatsomeFPweredestroyed,evenvanished,theultrastructureofGBMbecameambiguous,themesangialcellsswelledandtheextracellularmatrixaccumulatedwhichcausedmesangialregiontoexpandintensively.Afterthetreatmentofpioglitazone,thethicknessofGBMingroupPIOdecreasedma