不同强度的运动对大鼠心肺耐力及pgc-1α通路的影响-effects of different intensities of exercise on cardiopulmonary endurance and pgc - 1α pathway in rats.docx

mRNAwasincreasedinexercisegroups,buttheproteinexpressionofsoleusPGC-1αwassignificantlyincreasedonlyinthemoderateintensitygroup.4)TheAMPKproteinexpressionofmyocardiumandsoleuswasnotdifferentamongtheexercisegroups,butthepAMPKandSIRT1proteinexpressionwereincreasedwiththeincreaseofexerciseintensity.5)MyocardiumNRF1mRNAexpressionwasincreasedinmoderateandhighintensitygroup,soleusNRF1mRNAofallthreeexercisegroupswerelowerthansedentarygroup,andsowasthemRNAexpressionofNRF2.6)ThemoderateandhighintensityexerciseinducedmyocardialERRαmRNAexpression,butitwasdecreasedinthelowintensitygroup.ThechangeofsoleusERRαwasdifferentwiththemyocardial,thehighintensitygroupwasthelowest.Conclusions:1)12weeksofexerciseinterventioncansignificantlyimprovetheCRF.Thepromotioneffectsofcardiorespiratoryfitnessinmoderateandhighintensitygrouparefaster,andpeakvalueappearinthesixthandeighthweekrespectively.Butthelowintensitygroupimprovedsignificantlyfromthetenthweek,andthepeakvalueappearsinthetwelfthweek.2)12weeksofexerciseinterventioncanreducetherangeoftheratsweightgainsignificantly.Highintensityexercisegaingreatereffectofweightcontrolandheartfunction.2)ThemolecularmechanismofexerciseimprovedCRFwasmainlybyincreasingtheexpressionofskeletalmusclePGC-1α,butnotmyocardium.AMPKactivationandtheexpressionofSIRT1wereincreasedinmoderateandhighintensitygroup,especiallyinmoderategroup.4)AMPK,SIRT1,PGC-1αmRNAexpressioninexercisegroupscantreflecttherespectivelevelsofproteinexpression,possiblelythetranscriptionregulationplaysanimportantrole.Keywords:cardiorespiratoryfitness;aerobicexercise;differentintensity;PGC-1α论文随机验证编号：BT612420424270VI目录1前言.................................................................................................-4-2文献综述.........................................................................................-4-2.1PGC-1α.....................................................................................................-5-2.1.1PGC-1α家族及其亚型........................................................