吡格列酮对高脂追赶生长大鼠glp-1分泌障碍的影响及二甲双胍对nci-h716细胞脂质代谢相关酶和胰岛素信号通路的影响-effect of pioglitazone on glp - 1 secretion disorder in high-fat catch-up growth rats and effect of metformin on lipid metabolism-related enzymes and insulin sign.docxVIP

独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到，本声明的法律结果由本人承担。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。保密□，在_年解密后适用本授权书。本论文属于不保密□。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录前言··················································································5材料方法与结果·····································································7讨论················································································15参考文献············································································19第二部分二甲双胍对NCI-H716细胞脂质代谢相关酶和胰岛素信号通路的影响23中文摘要············································································23ABSTRACT25前言················································································27材料方法与结果···································································29讨论················································································38参考文献············································································43综述·················································································48参考文献··············································································54致谢·················································································57第一部分吡格列酮对高脂追赶生长大鼠GLP-1分泌障碍的影响及其可能机制中文摘要目的：观察高脂追赶生长对GLP-1分泌障碍的影响及给予吡格列酮干预后的影响，并探讨其可能机制。方法：72只6周龄Sprague-Dawley雄性大鼠，适应性喂养1周，随机分为：正常对照组（normalcontrolgroup，NC，n=36）和追赶生长组（catch-upgrowth，CUG，n=36）。NC组给予正常普通饮食喂养6周；CUG组给予普通饮食喂养6周，但进食量为NC组同体重大鼠进食量的60%。6周后，NC组随机分为正常对照组（n=12）、高脂组（highfat，HF，n=12）；CUG组随机分为追赶生长组（n=12）、追赶生长加吡格列酮干预组（catch-upgrowthwithpioglitazone，CUGP，n=12）。NC组予以普通饲料，自由摄食8周；HF组和CUG组给予高脂饲料，自由摄食8周；CUGP组给予高脂饲料，自由摄食8周，同时给予吡格列酮（100mg/kg）灌胃干预，每日一次。NC组、HF组、CUG组给予等量生理盐水灌胃处理。6周末和14周末分别取部分大鼠进行以下检测：（1）采用生化及ELISA法检测血清游离脂肪酸、甘油三酯、GLP-1水平；（2）采用高胰岛素-正糖钳夹实验测定葡萄糖输注率评价胰岛素敏感性；（3）采用免疫组化染色法观察回肠末端G