Chapter 8 DNA Fingerprinting and Forensic Analysis：8章DNA指纹图谱和法医分析.pptVIP

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Chapter 8 DNA Fingerprinting and Forensic Analysis：8章DNA指纹图谱和法医分析.ppt

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Chapter 8 DNA Fingerprinting and Forensic Analysis：8章DNA指纹图谱和法医分析

DNA Fingerprinting and Forensic Analysis What is a DNA fingerprint? Every cell of an individual carries a copy of the DNA a cell collected from a person’s skin or hair follicle contains the same DNA as from that persons heart tissue or white blood cells Order of base pairs in the DNA of every individual is different except identical twins How do we distinguish one person’s DNA from another? Don’t need to sequence all 3 billion base pairs of a person’s DNA to distinguish it from another person’s DNA Intron regions of DNA (junk DNA) contain sequences that are 20-100 bp in length that are repeated at different locations (loci) along the chromosome. CGGCTACGGCTACGGCTA (repeated 3 times at this location; at another location, it may be repeated 9 times) These sequences are called Short Tandem Repeats (STRs) or VNTRs STRs Each person has some STRs that were inherited from mother and some from father No person has STRs that are identical to those of either parent The number of repeats at each loci on chromosome is highly variable in the population, ranging from 4 to 40. The length of the DNA after cutting the chromosome with a restriction enzyme, and its position after electrophoresis will depend on the exact number of repeats at the locus The uniqueness of an individual’s STRs provides the scientific marker of identity known as a DNA fingerprint. In the United States the FBI has standardized a set of 13 STR assays (13 different locations on the chromosomes) for DNA typing, and has organized the CODIS database for forensic identification in criminal cases. The United States maintains the largest DNA database in the world: The Combined DNA Index System, with over 60 million records as of 2007. Preparation of a DNA fingerprintStep 1 Specimen collection blood, semen, etc easy to contaminate a DNA sample with DNA from other sources (bacteria, DNA of person collecting sample) DNA is not stable for very long-it degrades sunlight heat moisture DNA fingerprinting is a comp