固醇调节元件结合蛋白1基因及其相关基因的表达规律分析-analysis of expression of sterol regulatory element binding protein 1 gene and its related genes.docxVIP

IIII五个基因在部分组织中表达的影响都显著。但营养水平对基因表达的作用因组织不同而不 同。对 SREBP-1 是在小脑、皮脂、回肠、肝、肾，对 HMGCR 是在回肠，对 IDI1 是在肝， 对 SC4MOL 是在背肌、肝、肾，对 LDLR 是在回肠。比较同一种日粮（15.43%CP 日粮） 的作用，发现其对 SREBP-1 在小脑、皮脂、回肠中的表达影响不同，说明日粮与猪群之 间存在互作。比较添加精氨酸与否（15.43%CP 和 15.28%CP+1%精氨酸日粮）的作用，发 现其对 SREBP-1 在小脑、回肠中和 SC4MOL 在肝中的表达影响不同，即加了精氨酸后显 著降低 SREBP-1 在小脑、回肠中的表达量，而增加 SC4MOL 在肝中的表达量。③组织差 异除对 LDLR 基因的表达影响不显著外，对其它五个基因表达的影响都达到了差异显著水 平，且 SREBP-1、HMGCR、IDI1 和 SC4MOL 四个基因都是肝中表达最高。④在消除猪群 与营养因素的影响之后，SREBP-1 基因与其它五个基因间的相关性没有特定的规律可循， 但是 HMGCR、IDI1 和 SC4MOL 三个基因在小脑、背肌、回肠和肾四个组织中的相关较 高。显然，对 SREBP-1 基因及其调控基因的上述研究，将有助于更好地认识脂肪代谢机 理，指导猪的遗传育种和饲养管理，同时提高对人类脂质代谢性疾病如糖尿病、高脂血症、 脂肪肝、肥胖等的认识并指导临床治疗。关键词：猪；脂肪代谢；基因调控；固醇调节元件结合蛋白 1；β-羟-β 甲基戊二酰辅 酶 A 还原酶；异戊烯二磷酸-δ-异构酶 1；固醇 C4 甲基氧化酶；硬脂酰辅酶 A 去饱和酶； 低密度脂蛋白受体IIIIIISTUDY ON THE EXPRESSION OF SREBP1 GENE AND ITS TARGET GENESABSTRACTFat affects the flavor of pork meat, and more importantly is related to people’s health. However, fat metabolism process and its regulatory mechanism are complicated and involve many factors, among which, as a critical nuclear transcription factor, sterol regulatory element binding protein 1(SREBP-1) plays an important role. SREBP-1 can bind to sterol response-elements which locats in the promoters/enhancers of target genes, and can activate target genes’ transcription. Our study focused on SREBP-1 gene. Firstly, we analyzed a GEO dataset to explore the target genes and pathways regulated by SREBP-1; then by an experiment we analyzed the effects of genetic and nutritional factors on mRNA expression of SREBP-1, and several of its target genes and the pathways.Microarray data analysis of fat metabolism regulated by SREBP-1cWe searched and got microarray dataset GSE9132 from NCBI GEO database, which associated with mouse fat metabolism. By significant difference analysis about expression and plathway, we got 460 significantly differently expressed genes related to SREBP-1c over-expression in adipocyte differentiation process, among which 271 genes were up-regulated genes and 189 down-