共振散射光谱法测定白杨素 蛇床子素 丹皮酚及核黄素的分析-analysis of determination of chrysin, osthole, paeonol and riboflavin by resonance scattering spectrometry.docxVIP

摘要摘要IIII呈现良好的线性关系(r = 0.9925)，检出限为 2.38×10-2 μg/mL。该法简便快捷、 灵敏度高，用于市售维生素 B2 药片中核黄素的测定，结果满意。关键词：共振光散射法；白杨素；蛇床子素；丹皮酚；核黄素；β-环糊精；茜素 红AbstAbstractIIIIIIAbstractResonance light scattering (RLS) is a new technology developed in 1990s for trace analysis. Comparing with other methods, the RLS method has higher sensitivity, better selectivity and more simplicity. With the development of the RLS technique, it has been widely used in the research and determination of proteins, nucleic acids, cells, medication, surfactants medication, inorganic particles and non-particle. To further study and develop new RLS systems and methods has already become one of the important study subjects.This thesis consists of five parts, the main contents are as follows:The first part introduces the theory, principle, quantitative basis of RLS and the common analytical methods of chrysin, osthole, paeonolum and riboflavin.The second part used RLS to detemination chrysin in Senmen Oroxyli, based on the enhanced effect with SDS-chrysin-Al3+ system, new method of determining chrysin was found. Under the optimum reaction conditions, the RLS intensity at 565nm was proportational to the concentration of chrysin, and the linear range of 0.05～2.8 μg/mL μg/mL with the detection limit of 2×10-4 μg/mL. The recoveries is between 92.1～101.3%. It has been applied on the determination of chrysin in Oroxylum indicum with satisfactory results.The third part used RLS to detemination osthole in Common Cnidium Fruit, based on the enhanced effect with β-CD-osthole-AR system, new method of determining osthole was found. Under the optimum reaction conditions, the RLS intensity at 365 nm was proportational to the concentration of osthole,and the linearrange is 0. 5～12.0 μg/mL, detection limit is 7.3×10-3 μg/mL, the recoveries isbetween 95.6～105.1%. It has been applied on the determination of osthole in Common Cnidium Fruit with satisfactory results.The fourth part used RLS to detemination paeonolum in Pharyngitis Tablets.