[优质文档]!!!抑郁症神经基础研究.pptVIP

* PURPOSE OF THE SLIDE Show that acute and chronic stress and acute and chronic pain can all contribute to decreased levels of BDNF mRNA levels in the rat hippocampus. KEY POINTS In a preclinical study, 74 young male Sprague-Dawley rats were subjected to either immobilization stress or injected with an inflammatory stimulus (formalin or complete Freund’s adjuvant). Hippocampal BDNF mRNA levels were quantified using ribonuclease protection assays. Acute stress (n=12) rats were immobilized in restraint bags once for 45 minutes, while chronic stress (n=12) rats were immobilized for 45 minutes once daily for 10 consecutive days. Acute inflammatory pain rats received a subcutaneous injection of 100 μL of 5% formalin into the plantar aspect of the right hind paw. Animals were decapitated either at 2 hours and 45 minutes (n=12), 6 hours (n=7), or 24 hours (n=7) after the initiation of the final treatment. Chronic inflammatory pain (n=12) rats received a subcutaneous injection of 50 μL of complete Freund’s adjuvant (CFA) into the plantar aspect of the right hind paw. Decapitation followed 10 days later. Rats experiencing acute and chronic stress and rats experiencing acute and chronic pain had significantly lower hippocampal BDNF levels than did controls, suggesting an inhibition in BDNF synthesis. Acute or repeated stress significantly decreased bilateral BDNF hippocampal mRNA levels, by 61% and 50%, respectively, vs the control animals. The largest decrease in BDNF mRNA (67% decrease vs control animals) occurred at two hours and 45 minutes post formalin injection. Note that BDNF mRNA levels remained decreased for as long as 10 days in rats receiving CFA injections. REFERENCES Duric V, McCarson KE. Hippocampal neurokinin-1 receptor and brain-derived neurotrophic factor gene expression is decreased in rat models of pain and stress. Neuroscience. 2005;133(4):999-1006. * PURPOSE OF THE SLIDE At this point, the underlying structural, functional, neuroendocrine, neuroim