海洋酸化及uv辐射对硅藻光合作用影响的分析-analysis of the effects of ocean acidification and uv radiation on diatom photosynthesis.docxVIP

中文摘要中文摘要关键词：海洋酸化; 硅藻；CCM; UV 辐射; 光合作用;IIAbstractAbstractIIIIAbstractAtmospheric CO2 concentration has been rising since the Industrial Revolution and is predicted to double by the end of this century. The increasing atmospheric CO2 and subsequent rise of pCO2 in seawater, alters the carbonate system and related chemical reactions, resulting in lower pH and higher HCO3- concentration, which is called ocean acidification (OA), thus affects the photosynthetic processes in phytoplankton in direct and/or indirect ways. Diatoms play a fundamental role in marine biological CO2 pump, accounting for 40% of the marine primary produciton, however, little is known about their physiological responses to OA. Other environmental factors, like solar UV radiation and nutritive salt, could also affect the photosynthetic responses of diatoms, the combination effects of these factors need to be explored. In this study, we used pH/pCO2 perturbation technique to cultivate three diatom species (Skeletonema costatum, Thalassiosira weissflogii and Thalassiosirapseudonana) under two different pCO2 levels, representing the atmospheric pCO2 of present and the year around 2100 (pCO2: 800 ppmv) to evaluate the physiological responses of the diatoms to OA, and revealed the combined effects of OA and UV or nutritive salt. The main results are as follows:After acclimation to high CO2/low pH for 25 generations or more, the specific growth rates of S. costatum and T. weissflogii were decreased by 21% and 19.4% respectively. No extracelluar carbonic anhydrase activity was examined in both diatoms under OA conditions, while the activity of intracelluar carbonic anhydrase was inhibited by 54.8% and 31.3% respectively. OA lowered the maximum photosynthetic oxygen evolution rate, reduced the inorganic carbon affinity and down-regulated the carbon concentrating mechanisms (CCMs) in both diatoms.Both high visible light and solar UV radiation could result in the photoinhibition of T. weissflog