高脂血症模型小鼠睾丸生精细胞凋亡的分析-analysis of apoptosis of testicular spermatogenic cells in hyperlipidemia model mice.docxVIP

electron density was found in cytoplasm of germ cells. Vacuolization and tight sediment were observed in cytoplasm of Sertoli cells. Basement membrane thickened and winkled. The number of Mi and lipid droplets decreased obviously, and Mi also exhibited vacuole degeneration and rER expanded in Leydig cell. Mi exhibited vacuole degeneration and rER was degranulated in endothelial cells of continuous capillary. Nosignificant degenerative changes in ultra-structure were found in MT group. ④ Germcell apoptosis was detected in the testis of all groups. There was significant increase of germ cell apoptosis rate in hyperlipidemic mice compared with control group and MT group. ⑤ Bcl-2 and Bax expressed in the testis of all group. Bcl-2 expressed mainly in cytoplasm of spermatocyte and spermatoblast, and Bax localised in cytoplasm of spermatogonia, spermatocyte, Sertoli cell and nuclei of round spermatoblast. The expressions of Bcl-2 significantly down-regulated and Bax up-regulated in hyperlipidemic group compared with control group. The increased Bcl-2 expressions and decreased Bax expressions were observed in the testis MT group compared with the hyperlipidemic group. Conclusion Enhanced germ cell apoptosis occurred in hyperlipidemic mice, which may indicate the main mechanisms involved in hyperlipidemia-induced testis damage. Bcl-2 and Bax play a role in germ cell apoptosis in hyperlipidemic mice. Melatonin’s ability to prevent testicular damage induced by hyperlipidemia was documented.key words: hyperlipidemia; germ cell apoptosis; Bcl-2; Bax; testis; ApoE-knockout英 文 缩 略 词 表ApoEapolipoprotein E载脂蛋白 E MTmelatonin褪黑素 CHOcholesterol血清胆固醇 LDL-clow density lipoprotein cholesterol低密度脂蛋白胆固醇 HDLhigh density lipoprotein高密度脂蛋白 MODmean optical density平均光密度 HEHematoxylin-Eosin苏木精-伊红 TEMtransmission electron microscope透射电镜 TUNELteminal deoxynucleotidyltransferase-mediated dUTP nick end labeling脱氧核糖核酸末端转移酶介导的缺口末端标记 Bcl-2B-cell lymphonma g