线虫发育及基因功能研究2(韩珉).ppt

Strategy: moving from RTK down: discovering SOS Son of Sevenless: hyperactive mutation Drosophila: F1 screen vs F2 screen Mike Simon : landmark modify screen SOS encodes an exchange factor for GTPase Moving from receptor down: biochemistry Discovery of GRB2 Discovery of GRB2: complementary works by biochemists and geneticists The sem-5 story - early 1992 The rest of the story is there in a rush and clash- mid 1993 Many Small GTPases have a conserved C-terminus Ras is lipid modified for its membrane binding Half way done The Ras effector The Ras effector domain is defined by GAP = Ras effec