三峡库区栽培重楼属药植物中薯蓣皂苷元含量测定.docVIP

三峡库区栽培重楼属药用植物中薯蓣皂苷元含量测定 　　摘要：目的 建立高效液相色谱法测定重楼中薯蓣皂苷元含量，考察不同产地和不同品种重楼属药用植物中薯蓣皂苷元的含量。方法 采用岛津Inertsil ODS-C18色谱柱（150 mm×4.6 mm，5 ?m），以乙腈-水（92∶8）为流动相，流速1.0 mL/min，检测波长203 nm，柱温30 ℃。结果 不同产地、不同品种的重楼属药材中均含有薯蓣皂苷元，渝北区的长药隔重楼含量最高（6.813 7 mg/g），城口县的狭叶重楼含量次之（5.758 4 mg/g），最低的为石柱县的滇重楼（1.952 2 mg/g），表明不同栽培品种重楼药材化学品质差异较大，可能与产地、品种及栽培技术等因素有关。结论 非药典品种长药隔重楼、狭叶重楼含量超过中国药典收载品种，具有较大的药用价值。 中国论文网 /1/viewhtm　　关键词：重楼；薯蓣皂苷元；高效液相色谱法；质量评价 　　DOI：10.3969/j.issn.1005-5304.2016.06.021 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2016）06-0080-04 　　Abstract： Objective To establish an HPLC method to determine the content of diosgenin in Paridis Rhizoma； To examine contents of diosgenin in different varieties of Paridis Rhizoma medicinal plants and from different habitats. Methods Shimadzu Inertsil ODS-C18 guard column （150 mm×4.6 mm， 5?m） was used in HPLC system； elution conditions were the mobile phase using 92% acetonitrile and 8% water （isocraticelution） at a flow rate of 1.0 mL/min with 203 nm detection wavelength. The temperature of the column oven was maintained at 30 ℃. Results Different varieties of Paridis Rhizoma medicinal plants and Paridis Rhizoma medicinal plants from different habitats contained diosgenin. The Paris polyphylla var. pseudothibetica in Yubei district had the highest content （6.813 7 mg/g）； Paris polyphylla var. stenophylla in Chengkou County had the middle content （5.758 4 mg/g）； Paris yunnanensis Franch in Shizhu county showed the lowest content （1.952 2 mg/g）. The results showed that the obvious difference of chemical quality between various cultivated varieties of Paridis Rhizoma medicinal plants， which might be related with origin， variety and the cultivation technology， and so on. Conclusion The diosgenin contents of Paris polyphylla var. pseudothibetica and Paris polyphylla var. stenophylla were higher than that of varieties recorded in Chinese Pharmacopeia. Paris polyphylla var. pseudothibetica and Paris polyphylla var. stenophylla had important medical value. 　　Ke