血清淀粉样P蛋白缺失对动脉粥样硬化形成和发展-第三军医大学学报.DOCVIP

血清淀粉样P蛋白缺失对小鼠动脉粥样硬化斑块形成的影响李小强，王尽淘，桂亚丽，唐富天，兰 天，温定文，吴 腾，王丽京（510006广州，广东药学院血管生物学研究所）[摘要]目的 观察血清淀粉样P蛋白（Serum Amyloid P Component）缺失对小鼠动脉粥样硬化形成的影响。方法 构建载脂蛋白E（Apolipoprotein E）和血清淀粉样P 蛋白（SAP）双基因敲除小鼠（ApoE-/-；SAP-/-）作为实验组，ApoE-/-小鼠作为对照组，两组小鼠各12只，组内雄雌小鼠各半，在小鼠第8周龄时，两组小鼠体质量约20 g，此时开始同时给予两组小鼠高脂饮食，于第16周龄时获取小鼠全血分离得到血清进行血脂分析，处死小鼠后获取其心脏，胸腹主动脉进行油红O染色并进行病理统计。结果 双基因敲除小鼠组的高密度脂蛋白胆固醇（HDL-C）明显高于对照小鼠（P＜0.01），低密度脂蛋白和高密度脂蛋白的比值和动脉粥样硬化指数（AI）小于对照组小鼠且差异显著（P＜0.01），而总胆固醇（TC），甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）浓度无显著性差异（P0.05）；主动脉根部切片和胸腹主动脉的油红O染色结果表明SAP缺失明显减轻了小鼠动脉粥样硬化斑块面积（P0.01）。结论 SAP缺失可能通过上调HDL从而抑制ApoE[关键词]血清淀粉样P蛋白；高密度脂蛋白；动脉粥样硬化[中图法分类号]R543.5The Role of Serum Amyloid P Component Deficiency on Atherosclerosis in MiceLi Xiaoqiang，Wang Jintao，Gui Yali，Tang Futian，Lan Tian，Wen Dingwen，Wu Teng，Wang Lijing（Vascular Biology Research Institute， Guangdong Pharmaceutical University， Guangzhou，510006， China）[Abstract] Objective To observe the role of SAP deficiency on Atherosclerosis（AS）formation in mice. Methods ApoE-/-；SAP-/- mice, as the experimental mice, were obtained from the F2 offsprings by crossing ApoE-deficient mice with SAP-null mice, ApoE-deficient among the littermates were genotyped as control mice，both groups contained 12 mice, in each group, 6 male and 6 female were included. At the age of 8 weeks, the average body weight of these mice reached to about 20 g, then feed these experimental and control mice with western diet till their age of 16 weeks, blood, hearts and thoracoabdominal aortas were collected，serum were isolated from the blood for serum lipid analysis. Meanwhile， hearts were used to make frozen sections for oil red O staining for determination of the AS plaque lesion area at the aortic root， the thoracoabdominal aortas were stained by oil red O for En face analysis. Results The HDL-C concentration of the double-knockout mice were much higher than that of control （P 0.01）， the LDL-C/HDL-C value and Atherogenic Index （AI） were inversely much lower than that of control mice（P 0.01）.How