蒙古羊bmpr-ib fshβ基因克隆与表达及卵巢组织差异表达基因研究-cloning and expression of bmpr - ib fsh β gene in mongolian sheep and study on differentially expressed genes in ovarian tissue.docx

下载文档 降价啦

3
0
约18.41万字
约 152页
2018-07-27 发布于上海
举报
版权申诉
保障服务

蒙古羊bmpr-ib fshβ基因克隆与表达及卵巢组织差异表达基因研究-cloning and expression of bmpr - ib fsh β gene in mongolian sheep and study on differentially expressed genes in ovarian tissue.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

蒙古羊bmpr-ib fshβ基因克隆与表达及卵巢组织差异表达基因研究-cloning and expression of bmpr - ib fsh β gene in mongolian sheep and study on differentially expressed genes in ovarian tissue

StudyonCloningandExpressionofBMPR-IB，FSH?GeneandDifferentiallyExpressedGenesofOvaryinMongolianSheepAbstractThereproductiontraitsofovinearegenerallydividedintoconceptionrate，prolificacyandlambssurvival，itisdirectlyrelatedtoproductioncostandefficiencyofsheepindustryandithasagreateconomicsignificance.TheMongolianSheepisoneoftheancientindigenoussheepbreedsinourcount可.UjumuqinsheepisagoodgroupofMongolianSheepthroughalongbreedingunderthelocalecologicalenvironmentandithasmanyfinecharaclerislics，itisespeciallyfamousforitshighdiseaseresistance，dressingpercenlageandreproductiveperformance.lnordertorevealitshighreproductiveperformanceandtomakeaneff运ctiveutilizationoftheMongolianSheeppreciollsresource，lhisresearchusedUjumuqinsheepastheexperimentmaterial.First，ascandidategenesforprolificacy，theBMPR-JBandFSH?genewerecJoned，sequenceanalyzedanddifferentiallyexpressedinovary，ulerus，oviduct，hypothalamus，pituitarytissuesofnormalandestrusphysiologicalstageofsingleMongolianSheep，latepregnancyphysiologicalstageofbiparousMongolianSheepbyrelativequantitativeReal-timeQlIantitalivePCR(RQ-PCR)Secondly，differentiallyexpressedgenesbetweenovariesfromsingleandbiparousMongoliansheepwereselectedbysllppressionsubtractivehybr叫izalion(SSH)techniqueandsomedifferentiallyexpressedgeneswerefurtherclonedandverifiedbysiliconcloningandRT-PCR.Givenalltheaboveresearch，importanttheoreticalbasiswereprovidedforimprovingfecundityandbreedingnewvarietiesofmeatsheepinourcountry，andlhefollowingmainlyresultswereobtained:TheBMPR-JBandFSH?genecDNAsequencewereamplifiedfromMongolianSheepovarytissuesbyRT-PCRrespectively.Thefull-lengthofMongolianSheepBMP-JBgeneis1567bp，includingcomplete1509bpopenreadingframe(ORF)andencoding502aminoacids.ThebaseswereAandcinthe746and1113sitesofcodingregion，notoccurredthesamemutations(A→G)and(C→A)ofBMPR-JBgeneinBooroolaSheepbythesequenceanalysisofsingleandbiparousMongolianSheepBMPR-JBgene.Thefull-IengthofMongolianSheepFSJ{，卢geneis1021bp，incllldingcomplele390bpORFandencoding129aminoacids.The?气AC