美洛昔康对犬软骨细胞影响的试验分析-experimental analysis of the effect of meloxicam on canine chondrocytes.docxVIP

目录中文摘要.....................................................................IAbstract...................................................................II第一部分：文献综述...........................................................11骨关节炎的概述.............................................................11.1骨关节炎的病因.........................................................11.2骨关节炎的治疗方法及研究进展...........................................22软骨细胞的特征.............................................................42.1软骨细胞与骨关节炎间研究的联系.........................................42.2骨关节炎病理状态下的软骨细胞...........................................62.3软骨细胞的获取方法.....................................................82.4软骨细胞的体外培养方法.................................................93美洛昔康的研究进展.........................................................93.1美洛昔康的药理作用....................................................103.2美洛昔康的临床应用....................................................114本课题的研究目的和意义....................................................11第二部分：试验研究..........................................................12试验一软骨细胞的原代培养及鉴定.............................................12引言.......................................................................121试验材料..................................................................131.1试验动物..............................................................131.2主要仪器..............................................................131.3主要试剂..............................................................132试验方法..................................................................132.1犬关节软骨细胞的分离..................................................132.2犬软骨细胞的冻存与复苏................................................142.3制备软骨细胞爬片......................................................142.4甲苯胺蓝染色..........................................................152.5Ⅱ型胶原免疫鉴定.......................................................153结果与分析................................................................153.1软骨细胞的生长状态..........