玫瑰遗传多样性的srap研究与品种指纹图谱构建-srap study on genetic diversity of rose and construction of its variety fingerprint.docxVIP

玫瑰遗传多样性的SRAP分析与品种指纹图谱构建showedthatthegeneticrelationshipbetweenRosarugosaandRosamultiflorawasthenearestofthethree.TheNei’sgeneticdiversityandtheShannon’sinformationindexofRosarugosawas0.2665and0.4033respectively.TheNei’sgeneticdiversitywas0.1225andtheShannon’sinformationindexwas0.1787in4wildgermplasms,itwaslargelylowerthanthecultivars(H=0.2684,I=0.4059),showedthatthecultivarshasmoredistinctvariationandthegeneticdiversityofcultivarswasmoreabundant.ByUPGMAclusteranalysis,the56materialscanbedividedinto3groupsobviously.The3groupswere:Rosarugosa、RosamultifloraandRosa、Rosaxanthina.The50Rosarugosacanbedividedinto3series:rugosaroseseries、Chinaroseseriesandrosebushseries,therugosaroseseriescanbedividedinto7sections,whichwasconsistentwiththeclassificationbasedontheplanttypeandtheflowertype.ThespeciesoriginshouldbeconsideredfirstlyintheclassificationofRoserugosa,thentheplanttypeandtheflowertype.ThreecoreprimerpairswereselectedtoestablishthefingerprintofRosarugusa,allthecultivarscanbedistinguishedbythecombinationofeach2primerpairs.Gel2.0wasemployedtoestablishthefingerprintof46cultivars，whenthecomparabilitycoefficientwas95%,thefingerprintcanidentifyallthesampleinthetest.Keyword:Rosarugosa;SRAP;geneticrelationship;geneticdiversity;classification;fingerprint符号说明bp:BasePair,碱基对cM：centimorgan，厘摩CTAB：HexadecyltrimethyAmmoniumBromide，十六烷基三甲基溴化铵ddH2O：DoubleDistilledWater，双蒸水DNA：DeoxyribonucleicAcid，脱氧核糖核酸dNTPs：Deoxy-ribonucleosideTriphosphate,三磷酸脱氧核糖核苷Fig.：Figure，图像min：Minute，分钟PCR：PolymeraseChainReaction，聚合酶链式反应PVP：Polyvinylpyrrolidone,聚乙烯基吡咯烷酮RNA：RibonucleicAcid，核糖核酸SDS：SodiumDodecylSulfate，十二烷基硫酸钠TEMED：N,N,N,N-Tetramethylethylenediamine，N,N,N,N-四甲基乙二胺UPGMA：UnweightedPair-GroupMethodwithArithmeticmeans，非加权组平均法W：watt，瓦特关于学位论文原创性和使用授权的声明本人所呈交的学位论文，是在导师指导下，独立进行科学研究所取得的成果。对在论文研究期间给予指导、帮助和做出重要贡献的个人或集体，均在文中明确说明。本声明的法律责任由本人承担。本人完全了解山东农业大学有关保留和使用学位论文的规定，同意学校保留和按要求向国家有关部门或机构送交论文纸质本和电子版，允许论文被查阅和借阅。本人授权山东农业大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或其他复制手段保存论文和汇编本学位论文，同时授权中国科学技术信息研究所将本学位论文收录到《中国学位论文