浅低温对大鼠脑缺血再灌注损伤glt-1 及凋亡表达的影响-effects of mild hypothermia on glt - 1 and apoptosis in rats with cerebral ischemia-reperfusion injury.docxVIP

下载本文档

7
0
约4.88万字
约 80页
2018-07-28 发布于上海
举报
版权申诉

浅低温对大鼠脑缺血再灌注损伤glt-1 及凋亡表达的影响-effects of mild hypothermia on glt - 1 and apoptosis in rats with cerebral ischemia-reperfusion injury.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

浅低温对大鼠脑缺血再灌注损伤glt-1 及凋亡表达的影响-effects of mild hypothermia on glt - 1 and apoptosis in rats with cerebral ischemia-reperfusion injury

目录中文摘要·····························································································1英文摘要······························································································4英文缩写······························································································8研究论文浅低温对大鼠脑缺血再灌注损伤GLT-1及凋亡表达的影响前言·······························································································9材料与方法·················································································10结果··························································································22附图·····························································································25附表····························································································57讨论·····························································································58结论·····························································································62参考文献·····················································································63综述浅低温在缺血再灌注损伤中脑保护作用研究进展·······················66致谢···································································································79个人简历···························································································80浅低温对大鼠脑缺血再灌注损伤GLT-1及凋亡表达的影响摘要目的：研究大鼠全脑缺血再灌注即刻开始的头部浅低温对GLT-1及凋亡表达的影响，来进一步探讨浅低温的脑保护机制，为低温疗法在临床中应用提供参考。方法：健康雄性wistar大鼠258只，通过随机数字法随机分为6组，具体如下：1)对照组（cotrol组，n=6）:不做任何手术操作，直接断头取材。2)假手术组（sham组，n=42）：只做暴露双侧椎动脉和颈总动脉假手术操作。3)凝闭双侧椎动脉组（VOA组，n=42）:凝闭大鼠双侧椎动脉48小时后，暴露两侧颈总动脉穿线，但不阻断血流。4)常温缺血再灌注组（NI/R组，n=42）：凝闭大鼠双侧椎动脉48小时后，暴露并夹闭两侧颈总动脉8分钟，然后恢复血流再通，期间保持海马温度及肛温在37±0.5℃。5)低温组缺血再灌注组（HI/R组，n=42）：凝闭大鼠双侧椎动脉48小时后，暴露两侧颈总动脉，并穿线备用。采用鼻咽腔降温法待海马温度降至33±0.5℃后夹闭两侧颈总动脉8分钟，然后恢复血流再通，维持头部浅低温（33±0.5℃），肛温维持在37±0.5℃，2小时后自然复温。6)生理盐水+低温缺血再灌注组（NS+HI/R组，n=42）：凝闭大鼠双侧椎动脉48小时后，暴露双侧颈总动脉，并穿线备用，缺血前30min右侧脑室注射生理盐水20μL，采用鼻咽腔降温法待海马温度降至33±0.5℃夹闭双侧颈总动脉，8min后恢复血流再灌注，维持头部浅低温（33±0.5℃），肛温维持在37±0.5℃，2小时后自然复温。7)DHK+低温缺血再