双生子脑瘫证候-基因组学分析-syndrome - genomics analysis of cerebral palsy in twins.docx

张天娥博士学位论文 双生子脑瘫证候-基因组学研究 张天娥博士学位论文 双生子脑瘫证候-基因组学研究 - - PAGE 4 - 。 Through the analysis of SNP and CNV in three pairs cerebral Palsy——normal twins, 411 CNV were obtained. The correlated genes were much more the genes of Signal Transduction, Signaling Molecules and Interaction, Immune System, and the biology process were centralized in intercellular communication, metabolism and development gene. Discussion 。 The study method of “Zheng-genomics” applied in twins “Zheng-genomics” in twins is feasible either on theoretical or practical research. The key technology depends on choosing typical twins. 8 The multiple analysis of data in one of chip We adopted four databases classified by GO to analyze the data and explore information, and compared the up and down regulation gene express. This method provide the choosing evidence for the disease characteristic gene and passageway. Our analytic result indicated that this method was feasible. C) The analysis of gene expression profiling that reflect the disease characteristics . Though the systemic data analysis and information exploration of differential gene expression in cerebral palsy, we discovered that the up-regulation genes mainly belong to some fields such as metabolism, reaction to biostimulation; but the down regulation genes mainly belong to some fields such as intercellular communication. With the further classification by GO, the down regulation genes were concentrated in the G —protein coupled signal transduction. The most representative biology passageway was nerves ligand—acceptor. A series of genes reflecte disease characteristic were found out. 。 Creatively investigated the genomics structure difference in monozygotic twin The related genes analysis showed that some genes were mainly influenced such as Signal Transduction, Signaling Molecules and Interaction, and Immune System; the biology process were centralized in intercellular communication, metabolism and development gene. The resultant concordance of t