山东省家兔皮肤真菌病病原its序列测定及遗传变异研究-its sequence determination and genetic variation of dermatomycosis in shandong province.docxVIP

下载本文档

6
0
约6.17万字
约 62页
2018-07-30 发布于上海
举报
版权申诉

山东省家兔皮肤真菌病病原its序列测定及遗传变异研究-its sequence determination and genetic variation of dermatomycosis in shandong province.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

山东省家兔皮肤真菌病病原its序列测定及遗传变异研究-its sequence determination and genetic variation of dermatomycosis in shandong province

PAGE PAGE 4 山东省家兔皮肤真菌病病原 ITS 序列测定及遗传变异分析 isolated strains of the variation and genetic evolution relationship was discussed through cloning, sequencing to dermatophyte ITS gene, and then we acquired the satisfactory results by doing the tolerance detection of isolated strain. This research contains three parts: Firstly, design of universal primers based on specific rDNA sequences of dermatophytes can detect the known bacterial strain and other microorganisms by PCR. The PCR products are cloned and sequenced. PCR technology which is high sensitivity, speciality and rapidity has been used for dermatophytes identification. The results reveal that the detected and known bacterial strains were amplified a specific sequence of 650bp-770bp, but the other microorganisms , rabbit somatic cells and health rabbits were not. In addition, the detectablerate of PCR is higher than the method of conventional isolation and culture identification(82.8% and 60.55%). Then nine strains of rabbit dermatophyte were isolated and identified from major rabbit-farm in Shandong province, concluding 6 strains of Trichophyton mentagrophytes, 2 strains of Microsporum gypseum and 1 strian of Microsporum canis. Secondly, universal primers are designed according to conserved sequence internal transcribed spacers of dermatophytes, 16 strains of dermatophyte collected from major rabbit- farm in Shandong province were cloned and sequenced on ITS area. The result displays that the sequence of 5.8S rDNA in different pathogenic bacteria is highly conserved, but the variability of ITS is higher. Alignment analysis indicated that the length and the base composition of ITS1 were more variable than that of ITS2 among the species. Whereas in any species no difference was shown in the length of ITS1 and ITS2. The base composition was shown faintly diverse, so, rabbit dermatophyte were identified based on this area. This study confirmed that target sequence of PCR detected special primer by rabbit dermatom