双水相体系相平衡数学模型应用分析-application and analysis of phase equilibrium mathematical model for two-phase system.docx

AbstractAqueoustwo-phasesystem(ATPS)isaindofliquid-liquidsystem,thisindofsystemformwhenthesoluteisdissoledintothesolentandforcedthesolutiontoseparateintotopandbottomphases,aqueoustwo-phaseextraction(ATPE)isaindofextractiontechnoloybasedontheATPStheory.omparinwiththetraditionalliquid-liquid(oranicsolent/water)extractiontechnoloy,ATPEhasmanyadantaessuchasmildconditions,easyoperation,lowcostandsoon,soithasbeenwildlyusedintheresearchofseparationofdifferentindsofbiomolecularsinthepastseeraldecades.Inthiswor,wecarriedoutourresearchaboutthewaytoobtaintheinformationofphasediaraminATPSincludinbinodalcureandtielinedataandherearetheresearchresultsTheexperimentalbinodaldataofinestiatedATPSsincludinpolyethylenelycol(PEG)/phosphatebuffer(Kpi)systems,PEG/sodiumcitratesystemsandPEG/nonionicsurfactantsystemswasobtainedaccordintotitrationmethod.Theexperimentalbinodaldatawerecorrelatedbydifferentempiricalequationsforbinodalcureandbycomparinthealueofstandarddeiation(SD)ofeachempiricalequation,thefittestequationforbinodalcureineachATPSwasobtained.Accordintothe0.52fittinresults,theempiricalequationforbinodalcureW1=exp(a+bW2+cW2+dW2)hasmoreeneralapplicabilityforpolyethylenelycol(PEG)/phosphatebuffer(Kpi)systems,butthefittestempiricalequationsforothersystemsshowedotherness.BycombinindifferentempiricalequationsforbinodalcurewiththeleerruleofATPS,correspondinnonlinearequationsforeachempiricalequationwereobtainedandtheywerecorrelatedwiththeconcentrationsofeachcomponentinfeedsamplesandtheexperimentalaluesofolumeratiooffeedsamplestocalculatetheliquid-liquidequilibriumdataoffeedsamples(thecompositionsoftopandbottomphases).BycorrelatintheOthmer-TobiasandBancroftequationswithcalculatedliquid-liquidequilibriumdata,thelinearbbttbbdependencyofplotln((1-W2)/W2)aainstln((1-W1)/W1)andln((1-W2)/W2)aainstttln((1-W1)/W1)wereobtained,theresultsindicatedthatthemethodwhichcombinesempiricalequationswithleerruleofATPStocalculateliquid-liquidequilibriumdataisdependableandaccurate.Accordintothefittestempiricalequ