实验性矽肺模型大鼠肺组织中细胞因子表达及意义-expression and significance of cytokines in lung tissue of experimental silicosis rats.docxVIP

Objective:Toinvestigatethedynamicchangesofcytokinesinthelungtissuesofmodelratsafterone-timesilicainstillation.ToexploretheexpressionandsignificanceofIFN-γandIL-4inthelungtissueofmodelrats.TodiscusstheexpressionandpossibleeffectofNF-κBandTGF-β1inthelungtissueofmodelrats.Methods:Theexperimentalsilicosismodelinratswasestablishedbyintratrachealinstillationofsilica.Silicadustwithaone-time:animalsweresacrificedonthe1st,7th,14th,21st,28thand35thdayafterinstillation.TheexpressionofIFN-γandIL-4weredeterminedineighteenlungtissuesamplesofsilicaexposedratsatdifferentstageofsilicosiswhilelungtissuesamplesfromtwelvenormalratsweremeasuredascontrolbyimmunohistochemicalSABCmethod.TheexpressionofNF-κBandTGF-β1weredeterminedbyimmunohistochemicalS-Pmethod.Results:Dustwithaone-time:comparedwiththecontrolgroup,theexpressionofIFN-γsignificantlyenhanced(P0.05)andtheexpressionofIL-4obviouslyreduced(P0.05)inthelungofmodelratsonthe1st,7th,14thand21stday.Onthe28thand35thdayafterdust,theexpressionofIFN-γreducedandhadnosignificantdifferncewiththecontrolrats(P0.05),andtheexpressionofIL-4increasedobviouslyandwassignificantlyhigherthanthatincontrolgroup(P0.05).Onthe1st7thand14thdayafterdust,TheexpressionofNF-κBandTGF-β1inthelungofsilicosisratsincreasedobviouslycomparedwiththecontrolrats(P0.05).TheexpressionofbothNF-κBandTGF-β1inthelungofsilicosisratsdecreasedonthe21stdayandhadnosignificantdifferencewiththecontrolratsonthe28thday(P0.05).Onthe35thday,TheexpressionofNF-κBdecreasedobviouslyandhadsignificantdifferencewiththecontrolrats(P0.05),buttheexpressionofTGF-β1hadnosignificantdifferencewiththecontrolrats(P0.05).Conclusions:Intheearlystageofexperiment(1-21d),theexpressionofIFN-γwhichwasonekindofTh1cytokinesincreasedobviously,indicatingthatthecell-mediatedimmuneresponsebyTh1cytokineswaspredominantinthesilicosisrats.Inthelaterstage(28-35d),theexpressionofIL-4whichonekindofTh2cytokinesenhancedsignificantlyandtheexpressionofIFN-γreduced,suggestingthatthecell-mediatedimmuneresponsebegantodecreaseandhumo