石油烃降解菌降解性能 机理与应用分析-mechanism and application analysis of degradation performance of petroleum hydrocarbon degrading bacteria.docx

万方数据 万方数据 fermentation conditions for two strains were found, and the fermentation efficiency was increased. The effects of the system of W25 and W33 mixture in practical applications were verified.The laboratory orthogonal experiments were conducted on drill cuttings, and the effect of bacteria, fertilizers and surfactants on the compost was tested. The results showed that the bacteria had the biggest influence and fertilizers were the second. According to the results, pilot scale drilling cuttings biological compost treatment experiments were conducted by adding W25 and W33. After 73 days of test, the degradation percentages reached to 69.04%, while the control group was merely 17.75%. This indicated that the two strains had large potential for industrial applications. KEY WORDS: Petroleum hydrocarbon degrading bacteria, Brevibacillus panacihumi W25, Gordonia alkanivorans W33, Whole-genome sequencing, Drill cuttings treatment 目 录 第一章 文献综述...................................................................................................1 1.1 石油污染危害及在环境中的分布..........................................................1 1.1.1 石油污染的危害...........................................................................1 1.1.2 石油污染在环境中的分布...........................................................2 1.1.3 主要处置技术...............................................................................3 1.2 石油污染的生物降解..............................................................................6 1.2.1 石油降解微生物...........................................................................6 1.2.2 微生物降解主要影响因素...........................................................7 1.2.3 混合菌生物降解...........................................................................8 1.3 石油污染微生物降解途径分析..............................................................9 1.3.1 主要降解途径...............................................................................9 1.3.2 主要降解基因及功能......................................................