乙脑病毒感染的病毒学实验室诊断ppt课件.pptVIP

Virology and Laboratory Diagnosis of Japanese Encephalitis Virus Infection 乙脑病毒感染的病毒学实验室诊断;;Flaviviruses in China 中国黄病毒 Japanese encephalitis virus (JEV) 乙型脑炎病毒 Dengue viruses (DEN) 登革热病毒 Tick Borne encephalitis virus (TBE) (rare, near Russian border) 蜱传脑炎病毒（TBE）（罕见，于与俄罗斯边境处） There are 10 members of the JEV serocomplex, the main human pathogens of which are JEV, West Nile (WNV), Murray Valley encephalitis virus (MVE) and St. Louis encephalitis virus (SLEV). Of these, only JEV is found in China 乙脑病毒有10个成员，对人类致病的主要有乙脑病毒、西尼罗河病毒（WNV）、默里谷脑炎病毒（MVE），和圣路易斯脑炎病毒（SLEV）。其中，中国只发现乙型脑炎病毒;Characteristics of JEV infection and implications for laboratory diagnosis 乙脑病毒感染的特点和实验室诊断结果的解释 ;Diagnostic Detection Assays 诊断检测实验 Virus isolation – detects virus 病毒分离－检测病毒 Nucleotide amplification detection assays – detect viral RNA 核苷酸扩增实验－检测病毒RNA Serological assays – detect antibodies 血清学实验－检测抗体 Enzyme-Linked ImmunoSorbent Assay – antibody capture 酶联免疫吸附实验－抗体捕捉 IgM ELISA IgG ELISA Plaque reduction neutralization test (PRNT) 中和实验 Hemagglutination inhibition (HI) assay 血凝抑制实验 Complement fixation (CF) 补体结合实验;Theoretical Depiction of Human JE Viremia and Immune Response人乙脑病毒血症和免疫反应理论曲线;IgM detectable in serum csf by onset (99%) 出现症状后，血清和脑脊液中IgM的检出率99％ 6 exceptions serum of 800 cases, 1999-2002 1999-2002年，800个病例中6例血清学检测结果阴性 10 exceptions csf of 800 cases, 1999-2002 1999－2002年，800个病例中10例CSF结果阴性 IgG positive by day 7 post-onset 发病7天后IgG检测结果阳性 IgM persistence 1 Year IgM持续1年以上 Secondary flavivirus Infections or previous flavivirus vaccinations are problematic 再次感染黄病毒或既往接种过黄病毒疫苗会造成其它问题 ;Serological assays (IgM ELISA) more sensitive than virus isolation血清学检测 （IgM ELISA）较病毒分离敏感 ; Specimen type 标本类型 Cerebral spinal fluid 脑脊液 Serum 血清; Clinical spectrum of JE disease乙脑的临床特点;Long Term WN virus IgM activity in human serum (including in JE vaccinees) 西尼罗病毒血清学IgM的持久性;CSF preferable diagnostic specimen due to: 脑脊液作为更理想的诊断标