五脉地椒和石楠叶化学成分的提取分离及抑菌作用分析-extraction and separation of chemical constituents from zanthoxylum bungeanum and folium pyrrosiae and analysis of their bacteriostasis.docxVIP

AbstractTherearetworesearchingpintsinthisthesis:1)extractionandbactriostasisofThymusquinquelostatusCelakactiveconstituent;2)extractionandbactriostasiofPhotiniaserrulataL.ofleaves.Thisthesisdoesresearchonextraction,identificationofchemicalstructurofthesetwoplants.Themaincontentsincluding:ExtractThymusquinquelostatusCelakVolatileoilbySFE.Exploretemperature,pressureandtimehavewhatinfluenceonVolatileoilandextractionratio.Confirmthebestconditionofextractionis45℃，20Mpa，4h.DocomponentanalysisofThymusquinquelostatusCelakVolatileoilindifferentconditionsbyGC-MS,andfinddifferentconditionshavediiffrentinfluenceonThymusquinquelostatusCelakchemicalcomposition.ExtractThymusquinquelostatusCelakextratumbythemethodofcold-macerationwith75%ethanol.Theextratumissuspensionedbywater,andthenextractedbypetroleumether,chloroform,ethylacetate,n-butylalcoholandwaterinorder.Thefurtherresearchaboutitsexploitationwerecarriedoutaccordingtotheguidanceofbioassayingfromtheextract.Theresultsshowedextractofchloroform,ethylacetateandn-butylalcoholhavestronginhibitingeffectofthefivefungus,buttheextractofpetroleumetherwasweaker;Theextractofchloroformandethylacetatehavestronginhibitingeffectofthetwobacteriums,buttheextractofpetroleumetherandn-butylalcoholwasweaker.Get7compoundsfromextractofchloroform,ethylacetateandn-butylalcohol,andconfirmsthemas7,8-dihydroxy-6-methoxy-2-(4-methoxyphenyl)-2,3-dhydrochromen-4-on,dihydrokaempferol,ursolicacid,β-sitosterol，Linalool，1-octacosanol，sakuranetin.TheAntibacterialactivityresearchofLinalool，ursolicacid，dihydrokaempferol，β-sitostero，sakuranetinshowsthatsakuranetinisthebestone.Sakuranetinhasagreatereffectthancarbendazimonfivefungusesintheexperiment,andagreaterInhibitoryActivityonSclerotiniasclerotiorum,AcrospermumviticolaIKataandAlternariamalithanthiophanate-methyl.Whatismore,thedihydrokaempferol、ursolicacidandβ-sitosterolalsohaveabacteriostaticactivity.ExtracteffectivecontentsfromPhotiniaserrulataL.ofleavesbyAlcoholRefluxwith75%ethanolandextracttheextractumofPhotinia