下调mael和上调cdh1基因表达抑制肝癌hepg2细胞的迁移和增殖研究-down - regulation of mael and up-regulation of cd h1 gene expression inhibit the migration and proliferation of hepatocellular carcinoma hepg 2 cells.docxVIP

万方数据 万方数据 Abstract Objective: To investigate the migration, invasion and proliferation of HepG2 cell when up-regulating CDH1 gene and down-regulating MAEL gene. Methods: The siRNA of targeting MAEL and the saRNA of targeting CDH1 promoter were transfected into HepG2 cells with transfetion reagent. According to the different of transfection mixture cells were divided into five groups:CT group 、 NC group 、 778 groups 、 215 groups and 778+215 groups. qPCR was used to detect the expression of MAEL and CDH1, Western Blot was used to detect the expression of MAEL, E- cadherin, Caspase-3 and Bcl-2. MTT assay was used to detect the cell proliferation, scratch assay was used to detect the cell migration, the Transwell invasion assay was used to detect the cell invasion. Results: siRNA-778 and saRAN-215 was used to treat HepG2 cells.The qPCR showed that the siRNA-778 significantly reduced the expression of MAEL gene (p0.05) and the saRNA-215 was significantly upregulated CDH1 gene (p0.05). The Western blot shows that the expression of MAEL and Bcl-2 were significantly reduced (p0.05) and the expression of E-cadherin and Caspas-3 were significantly increased (p0.05). Both the cell proliferation and the scratch healing were significantly lower (p0.05), the number of HepG2 cells that passed through the basement membrane was significantly reduced (p0.05) than CT group, and CT group and NC group has no Statistical significance (p0.05). The proliferation, the migration and the invasion of the co-transfected cell were better inhibited than the siRNA-778 or saRAN-215 transfected cell (p0.05). Conclusion: The expression of MAEL genes can be effectively downregulated by siRNA-778, and the expression of CDH1 gene can be effectively upregulated by saRNA-215. The migration, invasion and proliferation of HepG2 cell were reduced. All these phenomena may related with the suppression of epithelial-mesenchymal transformation. And it may enhance apoptosis via Caspase-3/Bcl-2 signaling pathway.