太湖沉积物中微囊藻毒素降解菌的分离及其降解特性分析-isolation and characterization of microcystin - degrading bacteria in taihu lake sediment.docxVIP

TH菌降解MC的机理很可能与已报道的MC降解机理类似。关键词：微囊藻毒素，降解菌，降解途径，降解基因IIAbstractTheremovalofmicrocystininnaturalwaterismainlycausedbybiodegradation.Atpresent,numerousstudieshavefocusedonthemicroorganasimswhichareabletobiodegradeMC.However,thespeciesofmicrocystin-degradingbacteriawhichhavebeenreportedarerelativelyfew.OnlyoneMCdegradationpathwaywasreported.InordertofutherstudythebiodegradationpathwaysforMCLR,weneedtoisolatemoreMC-degradingbacteria.Inthispaper,theMCdegradationbacteriumwasisolatedfromTaihuLakesedimentusingMCLRasthesolecarbonandnitrogensource.Thestrainwasidentifiedbasedonmorphologicaland16SrDNAGenesequence.ThedegradationefficiencyofMCbythisstrainwasinvestigated.Furthermore,theinfluenceofenvironmentalfactors,suchastemperature、pHvalue,andnutritionsourcesonthisdegradationprocesswerestudied.Atlast,wepreliminarydeducedtheMCLRdegradationpathwaybystudyingthedegradationproductsanddetectingthedegradinggenecluster.Thespecificresearchcontentsandresultsareasfollows:ThemicrocystinsdegradationbacteriumwasisolatedfromTaihulakesediment.ThestrainTHisagram-negativebacteria.ThecolonymorphologyofstrainTHininorganicmediumiswhite,circular,edgetidy,withsmoothsurface.ThestrainTHisrod-shaped,bacteriacellswere1~3μminlengthand0.3~0.5μminwidth.16SrDNAsequencehomologyanalysisresultshowthatthebacteriaTHwasclosetotheRhizobiumsp.W29bacteria(KF560402).ThephylogenetictreeindicatethatTHwasbelongstotheRhizobiumgeneraunderα-proteobacteria.Whenincubatedat30℃andpH=7,thestrainTHcandegrading97%MCLRin12h.TemperatureinfluencedthedegradationofMCLR,strainTHshowthehighestefficiencyofMCdegradationwhenincubatedat30℃and35℃,whichcancompletelydegraded3μg/mlMCLRin12h.However,whenTHincubatedat20℃,theefficiencyofMCdegradationatthelaterperiodisslow.TheresultsofinfluencebypHvalueconfirmthatstrainTHshowthebestactivityatpHvalueof8and9,whilethedegradationratewereslowatotherpHvalues.ThedegradationreactionrateofthestrainTHwhichaddedcarbonsourceswas0.22h-1.Whilethedegradationreactionrateofthecontrolwas0.15h-1.Theresultconfirmsthatitwi