腺病毒介导n-bak基因转移对大鼠红核脊髓束的修复作用-repair effect of adenovirus-mediated n - bak gene transfer on spinal cord bundle of red nucleus in rats.docxVIP

第三军医大学博士学位论文 第三军医大学博士学位论文 英文缩写一览表 英文缩写 CNS 英文全称 Central nervous system 中文全称 中枢神经系统 SCI RST N-Bak Spinal cord injury Rubrospinal tract Neuron-specific isoform of the pro-apoptotic 脊髓损伤 红核脊髓束 神经元特异性 Bak AdV Bcl-2 family member Bak Adenovirus vector 腺病毒载体 RAD CPE PFU Recombinant adenovirus vector Cytopanthic effect Plaque-formating unit 重组腺病毒载体 细胞病理效应 空斑形成单位 CVL EGFP GFAP MCMV NT AST cDNA PCR RT-PCR Crude virus lysis Enhanced green fluorescence protein Glial fibrillary acidic protein Murine cytomegalovirus immediate early gene promoter Neurotrophin Astrocyte Complementary DNA Polymerase chain reaction Reverse transcription polymerase chain 粗制病毒裂解液 增强性绿色荧光蛋白 胶质原纤维酸性蛋白 小鼠巨细胞病毒即早基因 启动子 神经营养素 星形胶质细胞 互补脱氧核糖核酸 聚合酶链式反应 逆转录聚合酶链式反应 DEPC reaction Diethylprocarbonate 焦碳酸二乙脂 E1 ITR DAB PBS TE HE Early region 1 Inverse terminal repeat Diaminobezidin Phosphate buffered saline Tris-HCl and EDTA buffer Hematoxylin and Eosin 早期转录单位 1 末端反向重复序列 二氨基联苯胺 磷酸缓冲液 Tris 盐酸乙二胺四乙酸缓冲液 苏木精和伊红 1 PAGE PAGE 2 Amp Ampicillin 氨苄青霉素 Kan Kanamycin 卡那霉素 LB Luria-Bertani culture medium LB 培养基 DMEM Dulbecco’ s modified essential medium DMEM 培养基 Bcl-2 B cell lymphoma/leukemia-2 gene B 细胞淋巴瘤/白血病-2 基因 TUNEL Terminal deoxynucleotidal transferase-mediated dUTP-biotin nick end labeling 末端脱氧核苷酸转移酶介导 的 dUTP 缺口末端标记 Effects of adenovirus N-Bak gene transfer on rubrospinal tract injury in rats Abstract It is estimated that the annual incidence of spinal cord injury (SCI) is approximately 50 cases per million population and it is getting up in recent years. SCI primarily affects young adults. The costly expenses and handicap after the injury has been an overwhelming financial and social burden on the families and nations. This signals an urgent need for studies on its basical researcher and clinical treatments, so as to improve repair and recovery after SCI. The treatment of SCI is based on protection against delayed neuronal death. In recent experimental treatment, several strategies, such as neural nutritional fact