铜绿假单胞菌噬菌体pap1的基因组学分析一种由endonucleasev介导的细菌免疫新机制的发现与证实-genomic analysis of pseudomonas aeruginosa bacteriophage pap 1 discovery and confirmation of a new mechanism of bacterial immunity mediated by endoucleasev.docx

第三军医大学博士学位论文  PAGE 16 缩略语表 英文缩写 aa英文全称 amino acid中文全称 氨基酸HPLChigh-performance liquid chromatography高效液相色谱ddH2Odeionic distilled water去离子蒸馏水bpbase pair碱基对BLASTBasic Local Alignment Search Tool局部对比基本检索工具Dadalton道尔顿Vvoltage伏特DNAdeoxyribonucleic acid脱氧核糖核酸dNTPdeoxyribonucleoside triphosphate脱氧核苷三磷酸EBethidium bromide溴化乙锭EDTAethylenediamine tetraacetic acid乙二胺四乙酸Epeppendorf微量离心管ggram克hhour小时MSmass spectrometry质谱kgkilogram千克LBLuria-Bertani mediumLB 培养基mgmilligram毫克minminute分钟mLmilliliter毫升MOI NCBI nmmultiplicity of infection National Center for Biotechnology Information nanometer感染复数 美国国立卫生研究院 纳米ntNucleotide核苷酸 ODoptical density吸光度ORFopen reading frame开放阅读框PAGEpolyacrylamide gel electrophoresis聚丙烯酰胺凝胶电泳PCRpolymerase chain reaction聚合酶链式反应PEGpolyethylene glycol聚乙二醇PAPseudomonas aeruginosa铜绿假单胞菌PFUplague forming unit噬斑形成单位RNase Aribonuclease A核糖核酸酶 Arpmrotations per minute每分钟转速rRNAribosomal RNA核糖体 RNASDSsodium dodecyl sulfate十二烷基硫酸钠ssecond秒CDScoding sequence编码序列β-MEβ-mercaptoethanolβ-巯基乙醇dsDNAdouble-strand DNA单链 DNATAETris-acetate-EDTA bufferTAE 缓冲液TEMEDN,N,N,N-tetramethylethylenediamineN,N,N,N-四甲基乙二胺TM TrisTris-MgSO4 buffer Tris(hydroxymethyl) aminomethane含 Tris 和 MgSO4 的缓冲液 三（羟甲基）氨基甲烷tRNAtransfer RNA转运 RNAμLmicroliter微升RNAribonucleic acid核糖核酸PaPPseudomonas aeruginosa phage铜绿假单胞菌噬菌体μgmicrogram微克MWmolecular weight分子量 Genomic Analysis of the Pseudomonas aeruginosa Phage PaP1: Evidence of a Novel Immunity Mechanism of Bacteria Mediated by EndonucleaseⅤ Abstract Pseudomonas aeruginosa is a gram-negative opportunistic pathogen, frequently found in clinical treatment and resistant to many kinds of antibiotics. Pseudomonas aeruginosa is one of the main pathogens in hospital infection. Phages are viruses specifically infecting bacteria and ubiquitous in the biosphere. Estimations of phage numb