食品中农兽药残留及人工合成甜味剂标准化检测技术分析-analysis of standardized detection technology for veterinary drug residues and artificial sweeteners in food.docx

博士学位论文 博士学位论文 PAGE PAGE VI spiked in the blank tea, the results have shown good recovery on various spike standard levels. Their recoveries ranged from 56.4% to 125.5%, RSD were 1.1%~19.4%. A simple and sensitive high-performance liquid chromatography-mass spectrometry (HPLC-MS) method was established for the trace determination of aminoglycosides in animal-sourced food. Spectinomycin, hygromycin B, dihydrostreptomycin, streptomycin, amikacin, kanamycin, apramycin, tobramycin, gentamicin, and neomycin were tested as the target compounds. By ion-pair HPLC technique, the effective separation and determination of aminoglycosides was achieved, while HPLC-MS was developed for the confirmation of veterinary drug residues. The detection limits were about 20 μg/kg for spectinomycin, dihydrostreptomycin, streptomycin, amikacin, kanamycin, tobramycin, and gentamicin, 100 μg/kg for neomycin, apramycin, and hygromycin B. The method can meet the requirement of international standards for the inspection of aminoglycoside residues in animal-source food. For the pretreatment of the samples of aminoglycoside residues analysis, a new molecular imprinting polymer was synthesized. It had a high specific enrichmental ability to the target compounds. A highly sensitive method for determination of cyclamate in foods by ion-pair high-performance liquid chromatography-electrospray ionization mass spectrometry was developed and validated. The separation was achieved on a C8 column with 5 mmol/L tris(hydroxymethyl)aminomethane aqueous solution (pH=4.5) as mobile phase using an isocratic mode. The quantification of target compound was completed using selected ion recording (SIR) at m/z 178 obtained from ESI- mode. Tiopronin was used as internal standard for the quantification of cyclamate. The limit of detection was 5 ng/mL, the limit of quantification was 20 ng/mL. The method can be used to monitor effectively the content of the artificial sweetener in foods. The method has obvious mer