细胞周期调控中apcc降解机制的分析-analysis of apcc degradation mechanism in cell cycle regulation.docxVIP

万方数据 万方数据 ABSTRACT The discovery of protein degradation through the ubiquitin proteasome pathway in recent years,opened a new chapter on the mechanism research of Cyclin degradation by APC/C.Therefore,the research of Cyclin degradation mechanism by the ubiquitin proteasome pathway is of great significance. According to the literature, APC/Cdh1 plays a major role in G1 phase, with a highly active in order to guarantees the Cyclin dont accumulate too early and keeps cells in G1 phase.APC/Cdc20 plays a major role in M phase. In M phase,APC/Cdc20 initiates the metaphase-anaphase transition, promotes separation of sister chromatids and mitotic exit. In this paper, based on experimental phenomena, First of all, we used original model of CycB - Cdh1 - Cdc20, respectively to study the the influence of Cdh1 synthesis rate in the G1 phase and the cell cycle time. The study found that the increase or decrease of Cdh1 synthesis rate will bring G1 phase delay or shorten,but has no effect on M phase. secondly,we used the hybrid approach that combined the best features of continuous differential equations and discrete boolean networks. The concentration of Cyclin are tracked by piecewise linear differential equations for Cyclin synthesis and degradation., the transcription factors TFB whose activities are represented by discrete variables (0 or 1) and likewise for the activities of Cdh1 and Cdc20 complexes that govern Cyclin degradation. Then ,we build a discrete and continuous model of CycB-Cdh1-Cdc20,respectively to study the cell cycle under normal condition and the loss of Cdh1 in G1 phase as well the loss of Cdc20 in M phase. Found that when Cdh1 loss in G1 phase,the time of G1 phase is shortened, and Cdc20 missing in M phase induced the cells cannot be divided, this consequence is consistent with the experimental phenomena. KEYWORDS: CyclinB APC/C Cdh1 Cdc20 - III- 目 录 第一章 绪论 ...................................................................................................