B淋巴细胞刺激因子对慢性特发性荨麻疹患者产生抗FcεRI抗体和抗IgE抗体影响.doc

B淋巴细胞刺激因子对慢性特发性荨麻疹患者产生抗FcεRI抗体和抗IgE抗体影响 　　【摘要】 目的：探讨慢性特发性荨麻疹患者血清B淋巴细胞刺激因子（BlyS）对抗IgE抗体和抗FcεRI抗体的影响。方法：选取慢性特发性荨麻疹患者100例作为疾病组，选取同期体检健康者100例作为健康组。检测两组受试者血清BlyS、抗IgE抗体及抗FcεRI抗体水平；分离培养疾病组患者外周血中的B淋巴细胞，分为两份保存，在其中一份培养液中加入有效水平的BlyS，检测抗IgE抗体及抗FcεRI抗体水平，作为试验组，将另一份作为空白对照组。分析血清BlyS与抗IgE抗体及抗FcεRI抗体之间的关系。结果：疾病组血清BlyS、抗IgE抗体及抗FcεRI抗体水平明显高于健康组，差异具有统计学意义（P0.05）。试验组培养液中抗IgE抗体及抗FcεRI抗体水平明显高于对照组，差异具有统计学意义（P0.05）；试验组中抗IgE抗体及抗FcεRI抗体水平与BlyS呈正相关（r=0.765、0.722，P0.05）。结论：慢性特发性荨麻疹患者血清BlyS可刺激B淋巴细胞产生抗IgE抗体和抗FcεRI抗体，这可能与慢性特发性荨麻疹的发病机制有关。 　　【关键词】 慢性特发性荨麻疹； 免疫反应； B淋巴细胞刺激因子； 抗IgE抗体； 抗FcεRI抗体 　　【Abstract】 Objective：To investigate the affect of the serum B lymphocytes stimulating factor（BlyS） on the anti-IgE antibody and anti-FcεRI antibody of the patients with chronic idiopathic urticaria. Method： 100 cases of patients with chronic idiopathic urticaria were selected as disease group， 100 cases of healthy people in the same period were selected as the healthy group. The serum level of the BlyS， anti-IgE antibody and anti-FcεRI antibodies were detected； the B lymphocytes from the peripheral blood of the disease group patients were isolated and cultured， and divided into two shares， add BlyS in one share as the experimental group，another share as blank control group，the level of the anti-IgE antibody and anti-FcεRI antibodies were detected. The relationship among the BlyS，anti-IgE antibody and anti-FcεRI antibodies were analyzed. Result：The serum levels of the BlyS，anti-IgE antibody and anti-FcεRI antibodies of the disease group were significantly higher than the healthy group，the differences had statistical significance （P0.05）. The levels of the BlyS，anti-IgE antibody and anti-FcεRI antibody of the experimental group were significantly higher than the control group， the differences had statistical significance（P0.05）. The levels of the anti-IgE antibody and anti-FcεRI antibodies of the experimental group were positively correlated with the BlyS （r=0.765，0.722，P0.05）.Conclusion：BlyS can stimulate B