60Coγ辐射对杨树生理指标影响.docVIP

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60Coγ辐射对杨树生理指标影响

60Coγ辐射对杨树生理指标影响   摘要 分别对鲁山杨和圣山杨的种子进行不同60Co-γ射线辐射剂量的照射,得到M1代和扦插后的植株,测定其生理指标,结果表明:随着60Co-γ射线辐射剂量的增加,叶片中CAT活性、可溶性蛋白含量与SOD活性均表现出先升后降的趋势,MDA含量表现为先降后升的趋势。可溶性蛋白含量、SOD活性的峰值均在50 Gy时出现;CAT活性的峰值鲁山杨在100 Gy时出现,圣山杨在200 Gy时出现;MDA含量最大值在250 Gy时出现。综合分析表明高剂量60Co-γ处理会显著降低植株的抗性。   关键词 杨树;辐射;CAT;可溶性蛋白;MDA;SOD   中图分类号 S792.11 文献标识码 A 文章编号 1007-5739(2012)03-0242-01      Effect of Irradiation of 60Co-γ Ray on Physiological Indexes of Poplar   WANG Rui-jing REN Jun-zhan ZHAO Nan LI Lin FU Rui WANG Zhun ZHAO Lei-sheng ZHAO Dong-xia   (Jiyuan Forestry Bureau in Henan Province,Jiyuan Henan 459000)   Abstract The seeds of Populus× liaoningensis Z.Wang et H.D.Chen cv.nov and Populus × gaixianesis Z.Wang et H.D.Chen sp.cv were respectively irradiated by 60Co-γ with different doses,the M1 and cutting plants were obtained. Their physiological indexes were investigated,the results were as follows:CAT activities,soluble protein contents and SOD activities of leaves increased firstly,followed by decline with the enhance of irradiation doses.Whereas,the opposite change trends were observed in MDA content. The maximum values of soluble protein contents and SOD activity occurred under treatment of 50 Gy,while,CAT activity of Populus × liaoningensis Z.Wang et H.D.Chen cv.nov reached its maximum value under 100 Gy,the one of Populus × gaixianesis Z.Wang et H.D.Chen sp.cv was under 200 Gy.MDA contents of two poplars were the highest under 250 Gy. The results indicated that high irradiation doses decreased poplar resistance significantly.   Key words poplar;irradiation;CAT;soluble protein;MDA;SOD   杨树是杨柳科(Salicaceae)杨属(Po1ulus)树种的统称。杨属包括大叶杨派(Leucoides)、黑杨派(Aigeiros)、青杨派(Tacamahaca )、白杨派(Populus)和胡杨派(Turanga)等5个派,世界上分布的种数超过100种。在世界杨树的分布中,我国处于中心,杨树中的5个派在我国均有一定范围的分布,共有53种,其中特有种达到35种。   近年来,我国在果树、农作物及花卉等方面的辐射育种研究进展较大,取得的成果比较显著,但有关树木辐射育种的研究报道不多[1-3]。笔者选择不同剂量的60Co-γ射线分别对鲁山杨和圣山杨种子进行照射,测定其叶片中的CAT、可溶性蛋白、MDA及SOD等几项指标,以研究不同60Co-γ射线辐射剂量对M1代生理指标的影响。现将试验情况总结如下。   1 材料与方法   1.1 材料   试验材料为鲁山杨(Populus × liaoni

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