Apelin―13对脑缺血再灌注损伤作用初步研究.docVIP

Apelin―13对脑缺血再灌注损伤作用初步研究 　　【摘要】 目的：研究Apelin-13对缺血/再灌注小鼠脑损伤的作用，并初步探讨其对自噬性细胞死亡的影响。方法：取健康雄性CD-1小鼠，随机分为假手术（sham）组、生理盐水（Saline）组、Apelin组；Apelin组和Saline组首先建立脑缺血再灌注（MCAO/R）模型，再灌注即刻给予同侧侧脑室注射Apelin-13或等量生理盐水，然后在再灌注48 h后，进行神经功能方面检测，同时利用2，3，5-氯化三苯基四氮唑（TTC）染色评估脑梗死体积；缺血再灌注24 h和48 h后，采用West-blot法检测各组自噬相关蛋白LC3的表达情况。结果：Apelin-13可显著降低小鼠脑缺血再灌注后神经功能缺损症状，减少脑梗死体积，减低自噬相关蛋白LC3的表达及LC3-Ⅱ/LC3-Ⅰ的比值。结论：Apelin-13对小鼠脑缺血再灌注损伤有一定的保护作用，抑制神经细胞的自噬性死亡可能是其作用机制之一。 　　【关键词】 Apelin-13； 脑缺血再灌注模型（MCAO/R）； 自噬； LC3 　　The Effect of Apelin-13 on Mice after Middle Cerebral Artery Occlusion-Reperfusion Injury/KUAI Jin-xia，BAO Hai-jun，LI Zhou-ru，et al.//Medical Innovation of China，2016，13（20）：018-021 　　【Abstract】 Objective：To observe the effect of apelin-13 on the brain injury induced by middle cerebral artery occlusion-reperfusion（MCAO/R），and to explore the relationship between apelin-13 and autophagy in MCAO/R. Method：A total of 48 mice were randomly divided into sham，saline and apelin-13 groups.Firstly，mice s MCAO/R model was established using the suture’s method.After MCAO/R， Apelin-13 and saline groups were pretreated with an immediate injection of Apelin-13 and saline into the mice brain lateral ventricle.The effect of neurological deficit scores and the cerebral infarct volume was measured with TTC at 48h after MCAO/R.At last，to determine the role of Apelin-13 on the brain injury induced by MCAO/R，the autophagy associated proteins LC3 were also assessed with western-bloting.Result：Apelin-13 can significantly decreased neural dysfunction and cerebral infarct volume of mice after MCAO/R 48 h.Inadditionally，Apelin-13 also reduce the amount of protein LC3 expression and down-regulated protein LC3-Ⅱ/LC3-Ⅰratio，at 24 h and 48 h after MCAO/R.Conclusion：Apelin-13 preconditioning has obvious protective effect on mice after MCAO/R，and the mechanism may possibly by suppressing neuron autophagy. 　　【Key words】 Apelin-13； MCAO/R； Autophagy； LC3 　　First-author’s address：Xuzhou Medical University，Xuzhou 221002，China