Ang1对高糖中培养RF6A影响.docVIP

Ang1对高糖中培养RF6A影响 　　【摘要】 目的 探讨血管生成素-1（angiopoietin-1，Ang-1）对高浓度葡萄糖中培养的猴脉络膜-视网膜内皮细胞（RF/6A）的保护作用。方法 RF/6A分3组培养：对照组（DMEM培养基含25 mmol/L葡萄糖）、高糖组（DMEM培养基含40 mmol/L葡萄糖）、Ang-高糖组（DMEM培养基含40 mmol/L葡萄糖+0.25 mg/L Ang-1）。利用台盼兰染色法及MTT比色法检测体外培养的各种RF/6A存活率及细胞活力，利用western-blotting法检测各组suivivin表达。结果 1 d时各组细胞存活率及活力无差异。3、5、7 d时高糖组和Ang-高糖组细胞存活率及细胞活力均较正常组下降（P0.05），高糖组细胞存活率及细胞活力低于Ang-高糖组（P0.05）。5 d时，Ang-高糖组survivin表达明显较其他两组增多（P0.05）。 结论 高糖能降低RF/6A的存活率和活力，而Ang-1能明显增强高糖环境中RF/6A的存活率和活力,其机制可能与Ang-1上调凋亡抑制基因survivin的表达有关。 　　【关键词】 血管生成素-1；高糖； survivin表达 　　 　　Effects of angiopoietin-1 on RF/6A in high glucose environment 　　ZUO Zhong-fu,WANG Yi-jing,FENG Chuang,et al.Department of Pathophysiology,Liaoning Medical University,Jinzhou City,Liaoning 121001,China 　　【Abstract】 Objective To explore the damage of high glucose on rhesus macaque choroids-retinal endothelial cell（RF/6A）and also evaluate the protective effect and mechanisms of Angiopoietin-1(Ang-1) on RF/6A in this environment.Methods RF/6A were divided into 3 groups to be cultured:(1) control group (DMEM containing 25 mmol/L glucose); (2) high glucose group (DMEM containing 40 mmol/L glucose); (3) Ang-high glucose group (DMEM containing both 40 mmol/L glucose and 0.25 mg/L Ang-1).Each group were evaluated by measurement of MTT,trypan blue dye exclusion method at the 1 st,3rd,5th and 7th day respectively,and evaluated by western-blotting at the 5th day to determine the expression of survivin.Results At the 3rd,5th and 7th day,lower cell proliferation and livability in high glucose group and Ang-high glucose group were showed,compared with control group (P0.05),the same tendency was observed in high glucose group compared with Ang-high glucose group (P0.05).The western-blotting showed the expression of survivn in Ang-high glucose group was significantly higher than other two groups (P0.05).Conclusion High glucose can decrease the proliferation and livability of RF/6A while Ang-1 limits these damages in high glucose environment may by way