E选择素在大肠癌组织肿瘤细胞中表达及其临床病理意义.docVIP

E选择素在大肠癌组织肿瘤细胞中表达及其临床病理意义 　　【摘要】目的探讨E选择素蛋白在大肠癌组织肿瘤细胞中的表达及其临床病理意义。 　　方法运用免疫组织化学法，采用E选择素抗体检测116例大肠癌组织和57例癌旁正常肠黏膜组织E选择素的表达情况，分析比较E选择素在大肠癌肿瘤细胞和正常肠黏膜中的表达差异及其临床病理意义。 　　结果E选择素在大肠癌肿瘤细胞、血管内皮细胞均有表达，其在肿瘤细胞中阳性表达率（38.8%，45/116）明显高于癌旁正常肠黏膜腺体组织上皮细胞E选择素的阳性表达率（5.3%，3/57），两者差异有统计学意义（χ2=21.4335，Plt;0.001）。但肿瘤细胞E选择素阳性率在不同肿瘤直径、分化程度、浸润深度和有无淋巴结转移间差异无统计学意义（40.3% vs 37.0%、37.0% vs 42.9%、314% vs 44.6%、32.7% vs 43.8%，P均gt;005）。 　　结论大肠癌组织肿瘤细胞表达E选择素，但肿瘤细胞的这一分子特征在不同生物学行为的大肠癌病例间无显著差异。 　　【关键词】E选择素;大肠癌;免疫组织化学 　　中图分类号：R735.3+4 文献标识码：ADOI：10.3969/j.issn2016.01.002 　　【Abstract】ObjectiveTo investigate the expression of Eselectin protein in tumor cells of the colorectal cancer tissue and its clinical pathological significance. 　　MethodsThe protein expressions of the Eselectin in 116 cases of colorectal cancer tissues and 57 cases of normal intestinal mucosa tissue adjacent to carcinoma were detected by immunohistochemistry. Meanwhile， differences of expression and clinical pathological significance of Eselectin in tumor cells of the colorectal cancer tissue and normal intestinal mucosa were analyzed and compared. 　　Results The protein of the Eselectin expressed not only in vascular endothelial cells but also in colorectal cancer cells. The positive expression rates of Eselectin（38.8%， 45/116） in colorectal cancer cells was apparently higher than that（5.3%，3/57）in normal mucosa adjacent to carcinoma， so difference was statistically significant （χ2=21.4335，Plt;0.001）. However， differences of Eselectin in intestinal colorectal cancer cells and different tumor diameter， differentiation degree， infiltration depth and with or without lymph node metastasis was not statistically significant（40.3% vs 37.0%， 37.0% vs 42.9%， 31.4% vs 44.6% and 32.7% vs 43.8%， P all gt;0.05）. 　　ConclusionTumor cells of colorectal cancer can express Eselectin， but this molecular characteristic is not significantly different among different colorectal cancer cases with different biological behavior. 　　【Key words