FN和MMP14蛋白在鼻咽癌中表达及其临床意义.docVIP

FN和MMP14蛋白在鼻咽癌中表达及其临床意义 　　【摘要】目的探讨鼻咽癌组织中纤维连接蛋白（FN）和基质金属蛋白酶14（MMP14）的表达及其与临床病理特征的关系。方法选取56例鼻咽癌标本作为观察组，18例鼻咽炎标本作为对照组，采用SP免疫组织化学法检测两组标本中FN和MMP14的阳性表达情况，并分析二者的关系及其临床意义。结果基底膜FN在鼻咽癌组织中的表达低于鼻咽炎组织，间质FN在鼻咽癌组织中的表达高于鼻咽炎组织（P0.05），与淋巴结转移及临床分期相关（P0.01）；基底膜FN和 MMP14蛋白在鼻咽癌组织中的表达呈负相关（r=-0.478，P=0.000）。结论MMP14的高表达及FN的异常表达在鼻咽癌淋巴结转移及临床分期中可能具有重要作用。 　　【关键词】鼻咽癌；基质金属蛋白酶14；纤维连接蛋白 　　中图分类号：R739.6 文献标识码：ADOI：10.3969/j.issn2015.03.012 　　Expression and clinical significance of FN and MMP14 　　protein in nasopharyngeal carcinoma 　　TANG Wei，SUN Qi 　　（The Fourth Affiliated Hospital of Guangxi Medical UniversityLiuzhou 　　Worker’s Hospital，Liuzhou 545001，Guangxi，China） 　　【Abstract】ObjectiveTo investigate the expressions of fibronectin（FN） and matrix metalloproteinases14 （MMP14） in nasopharyngeal carcinoma and their relationship with clinicopathologic characteristics.Methods56 cases of nasopharyngeal carcinoma specimens were selected as observation group，and 18 cases of nasopharyngitis specimens as control group.SP immunohistochemical method was used to detect positive expressions of FN and MMP14 of specimens in both groups.Meanwhile，the relationship between the expressions and clinical significance was also analyzed.Results In nasopharyngeal carcinoma tissues，the positive expression of basement membrance FN was significantly lower than that in the nasopharyngitis tissues，while that of interstitial FN was higher than that in nasopharyngitis tissues（P0.05），but they had close relationship with lymphatic metastasis and clinical stage（P0.01）.Besides， the expressions of basement membrance FN was negatively correlated with MMP14 in nasopharyngeal carcinoma tissues （r=-0.478，P=0.000）.ConclusionHigh expression of MMP14 and aberrant expression of FN may play an important role in lymph node metastasis and clinical staging of nasopharyngeal carcinoma. 　　【Key words】nasopharyngeal carcinoma；MMP14；FN 　　鼻咽癌是我国南方及东南亚地区最常见的恶性肿瘤，其发生发展是一个涉及多基因、多途径和多阶段的复杂生物学过程[1]。纤维连接蛋白（FN）是基底膜及细胞外基质主要的糖蛋白成分，在肿瘤细胞的浸润