miRNA―26a在良恶性腹水鉴别诊断中意义.docVIP

miRNA―26a在良恶性腹水鉴别诊断中意义 　　[摘要] 目的 探讨miRNA-26a在良、恶性腹水鉴别诊断中的意义。 方法 收集北京大学深圳医院2011年9月～2014年3月门诊及住院患者60例，包括20例肝硬化腹水患者（肝硬化腹水组），20例结核性腹水患者（结核性腹水组），20例恶性腹水患者（恶性腹水组）。采用实时定量PCR（qRT-PCR）方法检测并比较各组血清miRNA-26a、甲胎蛋白（AFP）、糖类抗原125（CA125）的表达。 结果 恶性腹水组血清CA125表达高于结核性腹水组和肝硬化腹水组，但差异无统计学意义（P 0.05）。AFP、miRNA-26a在结核性腹水组及肝硬化腹水组表达差异无统计学意义（P 0.05）；AFP和miRNA-26a在恶性腹水组患者血清表达明显高于结核性腹水组和肝硬化腹水组，差异有统计学意义（P 0.05）。 结论 miRNA-26a水平将来有可能有助于良、恶性腹水的鉴别诊断。 　　[关键词] miRNA-26a；肝硬化腹水；结核性腹水；恶性腹水 　　[中图分类号] R442.5 [文献标识码] A [文章编号] 1673-7210（2014）08（b）-0051-04 　　Value of miRNA-26a level in benign or malignant ascites 　　LI Xi HE Min ZOU Bing▲ 　　Department of Gastroenterology， Peking University Shenzhen Hospital， Guangdong Province， Shenzhen 518036， China 　　[Abstract] Objective To investigate the value of serum miRNA-26a level in patients with benign or malignant ascites. Methods 60 cases in Peking University Shenzhen Hospital from September 2011 to March 2014 were included and divided into hepatic cirrhosis ascites group with 20 cases， tuberculous ascites group with 20 cases and malignant ascites group with 20 cases. miRNA-26a， AFP， CA125 level in blood serum were measured by qRT-PCR. Results The serum CA125 in malignant ascites group was higher than those in hepatic cirrhosis ascites group and tuberculous ascites group， but the difference was not statistically significant （P 0.05）. The differences of AFP， miRNA-26a between tuberculous ascites group and hepatic cirrhosis ascites group was not statistically significant （P 0.05）； the AFP， miRNA-26a in malignant ascites group were all higher than those in hepatic cirrhosis ascites group and tuberculous ascites group， the differences were statistically significant （P 0.05）. Conclusion Diagnosis of benign or malignant ascites may benefit from the measurement of miRNA-26a level. 　　[Key words] miRNA-26a； Hepatic cirrhosis ascites； Tuberculous ascites； Malignant ascites 　　在B超、MRI、CT、PET-CT等影像技术检查指导下，以及在临床各种常见生物化学检测指标（如肿瘤标志物等）帮助下，腹水患者的诊断准确性有了显著性提高。但是临床上仍有腹水患者诊