IgG在人肝细胞肝癌中表达及其临床意义.docVIP

IgG在人肝细胞肝癌中表达及其临床意义 　　【摘要】 目的：研究肿瘤源性IgG在人肝细胞肝癌中的表达和分布情况及其与临床病理学指标的关系。方法：分别应用免疫组化二步法和原位杂交检测60例肝细胞肝癌组织及其周围正常肝组织中IgG蛋白和IgG mRNA的表达，分析IgG的表达水平与9项肝细胞肝癌的临床病理学指标之间的关系。结果：肝癌组织中IgG的表达明显高于其周围正常肝组织，且两者表达程度比较差异有统计学意义（P=0.001）；IgG在肿瘤组织中的异常高表达与肿瘤分化程度和pTNM分期呈负相关（r=-0.357，P=0.005；r=-0.296，P=0.021），与血浆AFP浓度呈正相关（r=0.336，P=0.009），与年龄、性别、肿瘤包膜的完整性、肿瘤大小、肿瘤个数和一年内是否复发或转移无相关性（P0.05）。结论：肝癌细胞所产生的IgG与肿瘤的分化、pTMN分期相关，可能作为辅助肝细胞肝癌病理学诊断和预后评估的一项重要指标。 　　【关键词】 IgG； 肝癌； 临床病理学指标； 原位杂交； 免疫组织化学 　　【Abstract】 Objective：To investigate the expression and distribution of IgG in human liver cancer cells and its correlation with clinicopathological factors of hepatocellular carcinoma.Method：Fresh and paraffin-embedded tissues from 60 cases of HCC tumor with the matched normal liver specimens were detected by two-step immunohistochemistry and in situ hybridization for the expression of IgG protein and mRNA respectively. The relationship between cancer-derived IgG expression and 9 clinicopathological factors were analyzed statistically.Result：Signals of IgG protein in hepatocellular carcinoma cancer cells was significantly stronger than that in its corresponding normal tissues （P=0.001）； the over-expression of IgG was negatively correlated with several clinicopathological factors such as tumor differentiation grade and pTNM （r=-0.357，P=0.005；r=-0.296，P=0.021）， and positively associated with AFP concentration in serum （r=0.336，P=0.009）. However， no correlationship with age， gender， capsular infiltration，tumor size， tumor quantity and recurrence or metastasis of the disease within 1 year was found （P0.05）. Conclusion：Liver-cancer-derived IgG may has a profound impact on the differentiation and pTNM staging of liver cancer cell， and might be served as a tumour marker to help pathological diagnosis and evaluate the prognosis for patients. 　　【Key words】 IgG； Liver cancer； Clinical pathological factors； In situ hybridization； Immunohistochemistry 　　First-author’s address：Weifang Medicial University，Weifang 261053，