microRNA―31转基因小鼠脊髓损伤模型对胃肠动力障碍修复作用.docVIP

microRNA―31转基因小鼠脊髓损伤模型对胃肠动力障碍修复作用 　　[摘要] 目的 探讨microRNA-31对脊髓损伤后胃肠动力因素的作用。 方法 将36只microRNA-31转基因小鼠和36只FVB小鼠分别设为对照组和模型组，用Impactor M-Ⅲ脊髓撞击器建立小鼠脊髓损伤模型。用BBB运动功能评分评估小鼠下肢的恢复情况。HE染色观察脊髓组织的变化。用Real-time PCR法检测生长抑素受体（SSTR2） mRNA的表达，用免疫荧光法检测一氧化氮合酶（iNOS）蛋白和SSTR2蛋白的表达。 结果 造模后第3天、第7天和第14天，脊髓组织破坏、坏死、空泡形成，胶原纤维明显增加。造模后第14天，FVB模型组与对照组比较，SSTR2 mRNA的表达明显升高（P0.05），iNOS蛋白和SSTR2蛋白的表达量明显升高；microRNA-31转基因小鼠模型组与对照组比较，SSTR2 mRNA的表达明显升高（P0.05），iNOS蛋白和SSTR2蛋白的表达量明显升高；microRNA-31转基因小鼠模型组与FVB模型组比较，SSTR2 mRNA的表达明显降低（P0.05），iNOS蛋白和SSTR2蛋白的表达量明显降低。 结论 脊髓损伤后胃肠动力障碍的发生可能与SSTR2 mRNA的表达上调、SSTR2蛋白和iNOS蛋白的表达增高有关。高表达的microRNA-31可能与脊髓损伤后胃肠动力障碍的恢复有关。 　　[关键词] 脊髓损伤；胃肠动力因素；microRNA-31转基因小鼠；生长抑素受体（SSTR2）；一氧化氮合酶（iNOS） 　　[中图分类号] R651.2 [文献标识码] A [文章编号] 1673-9701（2016）13-0031-05 　　Repairing effect of microRNA-31 transgenic mice on gastrointestinal motility disorder in spinal cord injury model 　　WANG Dejun1 YAN Jun1 GAO Yuantao2 LI Pengfei3 WANG Chunfang3 　　1.Shanxi Medical University First School of Clinical Medicine， Taiyuan 030001， China； 2.Nanchang University Queen Mary School， Nanchang 330031， China； 3.Shanxi Medical University Laboratory Animal Center， Taiyuan 030001， China 　　[Abstract] Objective To discuss the effect of microRNA-31 on gastrointestinal motility factors after spinal cord injury. Methods 36 microRNA-31 transgenic mice and 36 FVB mice were divided into control group and model group， respectively. Spinal cord injury model was established by Impactor M-Ⅲ spinal cord striker. The recovery condition of lower limbs in mice was evaluated by BBB locomotion score， and the changes of spinal cord tissues were observed by HE staining. Expression of SSTR2 mRNA was detected by real-time PCR and expressions of iNOS protein and SSTR2 protein were detected by immunofluorescence method. Results At day 3， day 7， and day 14 of modeling， the spinal cord tissues were destroyed and necrotic with vacuolization， and collagenous fiber was markedly increased. At day 14 of modeling， when compared with the control gr