D二聚体检测诊断肺栓塞方法学研究与临床意义.docVIP

D二聚体检测诊断肺栓塞方法学研究与临床意义 　　【摘要】 目的 探讨血浆D-二聚体(D-dimer,DD)检测快速准确的方法和诊断肺栓塞的临床意义。方法 分别用胶体金双抗体夹心挪威Nycocard ReaderⅡ多功能全定量检测仪法、免疫比浊日本Sysmex CA1500全自动血凝仪法;手工目测定性法三种方法对42例确诊为肺栓塞的患者检测血浆中DD含量,记录结果进行比较。结果 42例肺栓塞患者DD检测胶体金法阳性42例,阳性率100%;免疫比浊法阳性42例,阳性率100%;与手工目测定性法比较差异有统计学意义(P0.05)。结论 胶体金法、免疫比浊法检测血浆D-二聚体结果准确、快速、便捷,作为肺诊断栓塞的首选过筛试验,值得推广应用。 　　【关键词】D-二聚体;肺栓塞;实验研究;临床意义 　　 　　methodology research and clinical significance of D-dimer examination diagnosis pulmonary embolism 　　 　　ZHOU Yu-huan,PENG He-ping,HE Wen,et al.Clinical lab in Karen Health and Hospital,Guangdong,Shenzhen 518102,China 　　 　　【Abstract】 Objective To discusses clinical practice of the blood plasma D-dimer(D-dimer,DD)examination fast accurate method and the diagnosis pulmonary embolism.Methods Using the colloid gold double immune body to contain filling separately Norwegian Nycocard ReaderⅡ;The multi-purpose entire quota instrumentation law,the immunity completely automatic blood congeal the meter law compared to muddy Japanese Sysmex the CA1500; The manual range estimate qualitative method three methods examine in the blood plasma to 42 example diagnoses for the pulmonary embolism patient the DD content,the recording result carry on the comparison.Results 42 example pulmonary embolism patient DD examination colloid gold law masculine gender 42 examples,masculine gender rate was 100%; Immunity turbidimetric method masculine gender 42 examples,masculine gender rate was 100%; There was the remarkable statistics significance with the manual range estimate qualitative method(P0.05).Conclusion colloid Jin Fa,the immunity turbidimetric method examination blood plasma D-dimer result accurate,is fast、 convenient,as the lung diagnosis embolism first choice sieving experiment,is worth promoting the application. 　　【Key words】D-dimer;Pulmonary embolism;Experimental study;Clinical significance 　　 　　作者单位:518102广东省深圳恒生医院检验科(周玉环 陈爱宝); 　　江西省吉安市第三人民医院检验科(彭和平 何文) 　　 　　 　　血浆-二聚体(D-dimer,DD)是一种纤维蛋白降解产物,正常人血中含量很低,遇微风栓形成的疾病可导致DD升高,肺