RECKMMP―14及VEGF在喉癌中表达及临床意义.docVIP

RECKMMP―14及VEGF在喉癌中表达及临床意义 　　[摘要] 目的 探讨RECK、MMP-14及VEGF在喉癌中的表达情况及临床意义。 方法 选择2013年8月～2015年1月辽宁省肿瘤医院选手术治疗的喉癌患者41例，分别取其喉癌组织和癌旁组织，另取喉息肉行手术切除患者的息肉组织作为对照。分别采用Realtime-PCR和Western blot检测各组织中RECK、MMP-14及VEGF mRNA和蛋白的表达情况，分析其与喉癌临床病理特征的相关性。 结果 MMP-14和VEGF在喉癌组织的表达明显高于癌旁组织及息肉组织（P 0.05），且其表达与病理分级、淋巴转移及临床分期有关（P 0.05）；RECK喉癌组织的表达低于癌旁和息肉组织，其与MMP-14（r = -0.812，P = 0.027）和VEGF（r = -0.907，P = 0.013）呈负相关。 结论 联合检测RECK、MMP14和VEGF基因对喉癌患者的诊断、转移及临床分期的确定具有重要意义。 　　[关键词] 喉癌；基质金属蛋白酶-9；基质金属蛋白酶-14；转移 　　[中图分类号] R736.1 [文献标识码] A [文章编号] 1673-7210（2016）01（b）-0085-04 　　[Abstract] Objective To investigate expression and clinical significance of RECK， MMP-14 and VEGF in laryngeal carcinoma. Methods From August 2013 to January 2015， in Tumor Hospital of Liaoning Province， 41 patients with laryngeal cancer and underwent surgical treatment were selected， the throat cancer tissue and para-carcinoma tissue were collected respectively， at the same time， polypus tissue from 15 patients with laryngeal polypus were collected as control. Realtime-PCR and Western blot were used to detect the organisations of RECK， MMP-14 and VEGF mRNA and protein expression respectively， the correlation of they and clinical pathological features of laryngeal cancer was analyzed. Results The expression of MMP-14 and VEGF in laryngeal carcinoma was significantly higher than those in para-carcinoma tissue and polypus tissue （P 0.05）， and their expression were related with pathological grading， lymphatic metastasis and clinical stages （P 0.05）； the expression of RECK in laryngeal carcinoma was significantly lower than those in para-carcinoma tissue and polypus tissue （P 0.05）， and it was negatively related with MMP-14 （r = -0.812， P = 0.027） and VEGF （r = -0.907， P = 0.013）. Conclusion The joint detection of RECK， MMP14 and VEGF genes has determining significance on diagnosis， metastasis and clinical stage for patients with laryngeal cancer. 　　[Key words] Laryngeal； Vascular endothelial growth factor； Matrix metalloproteina