CD105在急性白血病中表达及其临床意义.doc

CD105在急性白血病中表达及其临床意义 　　【摘要】 目的 探讨CD105在急性白血病骨髓细胞的表达及其意义。方法 分离骨髓单个核细胞,采用流式细胞仪(FCM)检测CD105的表达。结果 CD105在急性白血病骨髓细胞表达率[(30.39±8.69)%]明显高于对照组[(1.90±0.61)%](P0.05);且急性淋巴白血病表达CD105阳性率[(49.92±16.71)%]高于在急性髓系白血病的表达率[(22.21±9.95)%](P0.05);化疗缓解后CD105表达率[(3.99±1.45)%]比化疗前[(48.74±16.45)%]显著减低(P0.05);浸润组CD105表达率[(41.39±14.05)%]高于非浸润组[(21.06±9.14)%](P0.05)。结论 CD105在急性白血病细胞的异常表达,可能与白血病发病和预后有关。 　　【关键词】 CD105;白血病 　　 　　Expression and the clinical significances of CD105 in acute leukemia 　　 　　HE Jian-ye,ZHANG Qiu-tang.Children’s Hospital of Kaifengpost code,Kaifeng 475000,China 　　 　　【Abstract】 Objective This study was designed to explore the expression of CD105 in bone marrow cells from acute leukemia and its role in the development of acute leukemia. Methods The bone marrow mononuclear cells were separated and the expression of CD105 in them was detected by flow cytometry. Results Expressionof CD105 in acute leukemia bone marrow cells[(30.39±8.69)%] was significantly higher than those in control group[(1.90±0.61)%] (P0.05). Meanwhile, expression of CD105 in acute lymphoblastic leukemia cells [(49.92±16.71)%] was significantly higher than that in acute myeloid leukemic cells[(22.21±9.95)%](P0.05).Expression of CD105 was significantly reduced in remission patients after chemotherapy[(3.99 ±1.45)%] than those before chemotherapy[(48.74±16.45)%]. It was higher in patients with organ infiltration[(41.39±14.05)%] than that without infiltration[(21.06±9.14)%] (P0.05). Conclusions The abnormal expression of CD105 in acute leukemic cells may involve in the development and prognosis. 　　【Key words】 CD105;Leukemia 　　 　　作者单位:475000开封市儿童医院(何建业);郑州大学第一附属医院血液科(张秋堂) 　　 　　CD105是内皮细胞表面与增殖相关的膜抗原,同时又是调节转化生长因子-β (Transforming growth factor β,TGF-β)受体复合物成分之一,其主要作用为抑制TGF-β的生物学效应,促进血管新生,是新生血管的标志。然而,CD105在肿瘤细胞,如原发性或转移性黑色素瘤、卵巢癌、乳腺癌、卵巢的子宫内膜样癌细胞上弱表达[1]。国外研究表明,CD105在部分急性白血病细胞高表达[1],其临床意义需要进一步研究,国内未见报道。本文应用流式细胞术检测了急性白血病骨髓细胞中CD105的表达,并探讨了其临床意义。 　　1 资料和方法 　　1.1 一般资料 初治和复发急性白血病患者61例,其中ALL18例,AML43例;患者平均