三氧化二砷对食管癌细胞株EC―1生长抑制作用.docVIP

三氧化二砷对食管癌细胞株EC―1的生长抑制作用 　　【摘要】 目的：探讨三氧化二砷（（Arsenic trioxide，As2O3）对食管癌细胞株EC-1生长抑制作用及机制。方法：采用MTT法检测不同浓度As2O3 （0.5、1、2、4、8、16、32、64 μmol/L）在不同时间段（24、48、72 h）对EC-1细胞增殖的抑制率，计算半数抑制率（IC50）值。流式细胞术检测细胞凋亡及细胞周期变化。结果：三氧化二砷对食管癌EC-1细胞具有增殖抑制作用，呈剂量-效应和时间-效应关系（P0.05）。IC50约为18.74 μmol/L。As2O3可使细胞阻滞在G2/M期，使G0/G1期、S期细胞比例减少，增加细胞凋亡，差异具有统计学意义（P0.05）。结论： As2O3能抑制食管癌EC-l细胞株的增殖，具有明显的量效和时效关系。其机制可能与改变周期，增加其细胞凋亡有关。 　　【关键词】 食管癌EC-1细胞系； 三氧化二砷 　　Effets of Aresenic Trioxide on Esophageal Squamous Carcinoma Cell Line EC-1/GU Jian-fa，WANG Xiao-min，LU Ping.//Medical Innovation of China，2015，12（30）：116-118 　　【Abstract】 Objective： To investigate effects of arsenic trioxide （As2O3） on esophageal squamous carcinoma cell line EC-1. Method：The inhibition effect of As2O3（different doses of 0.5，1，2，4，8，16，32，64 μmol/L） on EC-1 cells proliferation at different time points（24，48，72 h） was detected by MTT assay，and the value of IC50 was calculated .Cell cycle change and cell apoptosis were detected by flow cytometric analysis （FCM）.Result：As2O3 was able to inhibit the proliferation of esophageal squamous carcinoma cell line EC-1，with the dose of As2O3 increasing and the effect time prolonging，the inhibition of cells proliferation was enhanced.The IC50 in EC-1 cells was 18.74 μmol/L. As2O3 could induced G2/M phase arrest and the reduction of the ratio of cells in G0/G1 and S phases expectively. As2O3 could enhance the radiation-induced apoptosis. Conclusion： The proliefration inhibition action of As2O3 on esophageal cell line Ec-1 depends on the concentration and the contact time. As2O3 hyperthermia can enhance the killing effect of radiation on tumour cells mainly through affecting the EC-l cell cycle， and the induction of apoptosis is the second reason. 　　【Key words】 Human esophageal carcinoma EC-1 cell line； Arsenic trioxide 　　First-author’s address：Zhengzhou Center Hospital Affiliated to Zhengzhou University， Zhengzhou 450007，China 　　doi：10.3969/j.issn.1674-4985.2015.30.038 　　中药砒霜的有效成份即为三氧化二砷（As2O3）