不同受精方式对单原核胚胎发育潜能影响.docVIP

不同受精方式对单原核胚胎发育潜能的影响 　　【第一作者简介】王珊珊(1986-)，女，实习研究员、硕士，主要从事生殖医学研究。 　　【摘要】目的:探讨常规体外受精（IVF）及卵胞浆内单精子注射（ICSI）后单原核胚胎的发育潜能，为提高卵子利用率探寻可行的方法。方法:回顾性分析3614个IVF周期和1306个ICSI周期的患者资料。按照年龄分为35岁3组，分析不同授精方式下女方年龄对单原核受精卵出现的影响，并进一步研究35岁以下患者中，不同授精方式对单原核胚胎发育潜能的影响。结果:在IVF和ICSI周期中，35岁组的单原核受精卵形成率显著高于30岁组(P0??05)。35岁以下女性患者中，IVF组单原核胚胎的囊胚形成率及优质囊胚形成率显著高于ICSI组（28??97% vs 12??12%，11??90% vs 0），并具有统计学差异(P0??05)。结论:女方年龄、授精方式可能与异常受精的发生有关。在行辅助生殖技术（ART）时应综合考虑这些因素，为临床提高卵子利用率提供新思路。 　　【关键词】体外受精；卵胞浆内单精子注射；单原核 　　Influence of different fertilization modes on the developmental potential of monopronucleus embryosWANG Shanshan, ZENG Huiming, ZHANG Ningyuan, SUN Haixiang△. Reproductive Medicine Center, Drum-Tower Hospital Affiliated to Nanjing University Medical School, Nanjing 210008, China 　　【Abstract】Objectives: To investigate the developmental potential of monopronucleus embryos in in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to explore a feasible approach for improving the utilization rate of oocytes. Method: Data of 3614 patients in IVF cycles and 1306 patients in ICSI cycles were retrospectively analyzed respectively, and divided into three groups according to age of patients, 35 years group. The impact of age on monopronucleus zygotes was analyzed in different fertilization modes and the influence of different fertilization modes on the developmental potential of monopronucleus zygotes was further studied in group of patients under 35 years old. Results: The formation rate of monopronucleus zygotes for the group 35 years was substantially higher than that in group 30 years (P0??05) in IVF and ICSI cycles. Among female patients under 35 years old, the blastocyst rate and high-quality blastocyst rate of embryos from monopronucleus zygotes in the IVF group were substantially higher than the ICSI group (28??97% vs 12??12%, 11??90% vs 0), with statistically significant differences (P0??05). Conclusion: The age of female patients and fertilization modes may be related to the occ