不同干燥方法对川麦冬甾体皂苷和高异黄酮影响お.docVIP

不同干燥方法对川麦冬甾体皂苷和高异黄酮的影响お 　　[摘要]该研究以川麦冬为研究对象，考察不同干燥方法对麦冬药材中化学成分的影响。取新鲜川麦冬块根粗粉，分别采用50 ℃烘干干燥、80 ℃烘干干燥、-70 ℃冷冻干燥3种方法进行干燥处理，应用HPLCDADELSDMSn多检测器串联技术，筛选鉴定干燥过程中变化显著的高异黄酮类和甾体皂苷类化学成分。结果显示：与新鲜麦冬药材的HPLC图相比，50 ℃烘干干燥最能保持药材固有化学成分；80 ℃烘干干燥、-70 ℃冷冻干燥方法则对化学成分影响较大；结合HPLCHRMSn数据，最终筛选了36个差异性成分，并鉴定了其中24个差异性成分（11个甾体皂苷和13个高异黄酮）的结构。结果表明，不同干燥方法对川麦冬中甾体皂苷和高异黄酮有明显影响；从成本、成分含量和实用性综合分析，50 ℃烘干干燥更有利于川麦冬药材中固有成分的保留，可以为川麦冬药材的产地干燥加工提供借鉴。 　　[关键词]川麦冬； 甾体皂苷； 高异黄酮； 干燥方法； HPLCUVELSDMSn 　　Influence of different drying methods on steroidal saponins and 　　homoisoflavonoids in Ophiopogon japonicus 　　WANG Fei1， SHANG Zhanpeng1， MA Zhiguo2， LIU Siyi1， WANG Zijian3， ZHANG Jiayu3*， LU Jianqiu4* 　　（1School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China； 　　2College of Pharmacy， Jinan University， Guangzhou 510632， China； 　　3Beijing Institution of Chinese Medicine， Beijing University of Chinese Medicine， Beijing 100029， China； 　　4Library of Beijing University of Chinese Medicine， Beijing 100029， China） 　　[Abstract]To study the influence of three different drying methods （including 50 ℃drying， 80 ℃drying and -70 ℃freezedrying methods） on steroidal saponins and homoisoflavonoids in Ophiopogon japonicus，a HPLCDADELSDMSn method was investigated to screen and identify the differential components Through comparing the HPLC chromatograms with that of fresh O japonicus， 50 ℃drying medicine was similar with fresh medicine whereas the other two drying methods had great influence on the components of O japonicus In this study， 36 differential components were screened， among which 24 constituents（13 homoisoflavonoids and 11 steroidal saponins） were identified via HPLCLTQOrbitrap MSAs a result， it was revealed that different drying methods had significant influences on the components of steroidal saponins and homoisoflavonoids Among them， 50 ℃drying method was the most suitable drying approach when the stability of components， cost and practicability were considered 　　[Key words]Ophiop