不同强度动训练对大鼠心室肌胶原纤维和Cx43Cx45影响实验研究.docVIP

不同强度动训练对大鼠心室肌胶原纤维和Cx43、Cx45影响的实验研究 　　摘 要: 目的:探讨有氧训练和无氧训练对大鼠心室肌胶原纤维形态结构和Cx43、Cx45分布和 表达的影响。方法:采用跑台训练方式,建立大鼠有氧运动和疲劳运动模型,采用Masson染 色法和免疫组织化学SABC法观察心室肌胶原纤维形态结构和Cx43、Cx45表达的变化。结果: 有氧训练组心室肌胶原纤维分布均匀,结构完整,Cx43、Cx45构型端?端连接多于侧?侧连 接且表达均显著高于安静对照组和疲劳训练组(P0.05)。疲劳训练组胶原纤维出现过 度增生, 扭曲和局部溶解现象,Cx43、Cx45构型改变,侧?侧连接多于端?端连接,且Cx43 、Cx45表达显著低于安静对照组和有氧训练组(P0.05)。结论:不同强度运动训练影响 大鼠心室肌胶原纤维组织结构,Cx43、Cx45的分布和表达,大强度疲劳训练可造成心室肌胶 原纤维病理改变,Cx43、Cx45构型和量的变化,推测与运动实践中室性心律失常及运动猝死 的发展、发生存在密切联系。 　　关键词:运动训练;免疫组化;心室肌;连接蛋白 　　中图分类号:G804.2文献标识码:A文章编号 :1007-3612(2009)06-0047-04 　　 　　Effects of Exercise for Collagen Fibers and the expression of Cx 43, Cx45 on Rats Ventricular myocardium 　　WANG You?hua,TIAN Zhen?jun 　　(Institute of Physical Education, Shaanxi Normal University,Xi an 710062, Shaanxi China) 　　Abstract: Objective To investigate the effects of aerobic training and training? induced fatigue for collagen fibers and Cx43, Cx45 of ventricular myocardium. Me thods Rat models of aerobic training and training?induced fatigue groups were e s tablished through running on an animal treadmill. Masson staining and immunohist ochemistry SABC were applied to observation collagen fiber tissue structure, dis tribution and the expression of Cx43, Cx45 in the ventricular myocardium. Result s In the aerobic training group, collagen fibers uniform arrangement and structu re integrity, distribution of Cx43, Cx45 that end?to?end fashion more than sid e? to?side orientation and the expression of Cx43, Cx45 in aerobic training groupwas higher than that in the control group and training?induced fatigue group si gnificantly (P0.05). In the ventricular myocardium of training?induced fat igue group, the collagen fibers were abnormally proliferated, twisted and locali zed dissolution, the distribution of Cx43, Cx45 that side?to?side orientationmore than end?to?end fashion and the expression of Cx43, Cx45 lowersignifican tly compared with control and aerobic training groups (P0.05). ConclusionsExercise training with differen