丙烯菊酯和胺菊酯与DNA相互作用光谱研究.docVIP

丙烯菊酯和胺菊酯与DNA相互作用的光谱研究 　　摘要:采用紫外和荧光光谱法,研究了丙烯菊酯和胺菊酯与单双链DNA、鸟嘌呤及鸟嘌呤核苷之间的相互作用。拟除虫菊酯农药可使DNA溶液的吸收光谱出现减色效应,但最大吸收波长无明显位移;不同浓度的DNA可导致农药荧光猝灭。还分别考察了离子强度、KI对农药-DNA体系的影响及农药对中性红-DNA体系的影响。结果表明:丙烯菊酯与ssDNA之间存在静电和沟槽两种作用方式,而胺菊酯与ssDNA之间主要是静电作用。鸟嘌呤和鸟嘌呤核苷与农药作用的紫外和荧光光谱研究表明,它们与农药之间主要以氢键相互作用,且鸟嘌呤核苷与农药的作用比鸟嘌呤与农药的作用要强。 　　关键词:丙烯菊酯;胺菊酯;DNA;作用方式;光谱法 　　Studies on the interaction of allethrin and tetramethrin with DNA by spectrometry 　　Zhaoyang-Wu1,Yin-Liu1,2,Chonglin-Luan2,Xiaohua-Jiang2 　　(1 State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry and Chemical Engineering , Hunan Univ , Changsha , Hunan 410082, China; 　　 2 Department of Fine Chemical Engineering, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, China) 　　Abstract: The interaction of allethrin and tetramethrin with single strand DNA (ssDNA),double strand DNA, guanine and guanosine were studied by UV and fluorescence spectrophotometry. Pyrethroid Pesticide brought about a hypochromic effect on the UV absorption spectrum of DNA, but the maximum absorption wavelength of DNA did not shift obviously; the addition of varied concentration of DNA caused the fluorescence quenching of pesticides. It was investigated that the effect of ionic strength and KI on the system of pesticide-DNA respectively as well as the effect of pesticide on the neutral red-DNA. The results indicated that the electrostatic binding and groove binding are the two major modes of the interaction between allethrin and DNA, while electrostatic binding mainly exists between tetramethrin and DNA. The spectra of UV and fluorescence of Guanine and guanosine interact with pesticides proved that the interaction between them and pesticide is hydrogen bonds chiefly, and guanosine interacts with pesticides stronger than guanine does. 　　Keywords: allethrin; tetramethrin; DNA; interaction; spectrometry 　　 　　 拟除虫菊酯是根据天然菊素化学结构仿生合成的一种广谱高效杀虫剂,是继有机氯杀虫剂、有机磷杀虫剂、氨基甲酸酯杀虫剂之后的一个新突破[1], 被广泛应用于蔬菜,水果,粮食,茶叶,烟草等作物的病虫防治,使用量逐年增加。拟除虫菊酯农药为神经毒物,对鱼类、蜜蜂和家蚕的毒性较高[2,3]