乌司他丁对侵袭性念珠菌感染小鼠肝脏线粒体功能保护作用.docVIP

乌司他丁对侵袭性念珠菌感染小鼠肝脏线粒体功能的保护作用 　　[摘要] 目的 探讨乌司他丁对侵袭性念珠菌感染小鼠肝脏线粒体功能的保护作用和可能机制。 方法 雄性昆明小鼠60只，经CTX预处理后腹腔注射白念珠菌混悬液，制作侵袭性念珠菌感染小鼠模型，随机分成对照组（n=20）、乌司他丁低剂量组（n=20）和乌司他丁高剂量组（n=20）。对照组给予抗真菌药物治疗，低剂量组给予抗真菌药物联合乌司他丁（1×104 U/kg）治疗，高剂量组给予抗真菌药物联合乌司他丁（5×104 U/kg）治疗，观察3组第7天生存率、第7天线粒体生成指标、第7天氮化应激指标和第7天肝脏病理。 结果 高剂量组第7天生存率显著高于对照组（70% vs 30%，P=0.011），第7天高剂量组PGC-1α mRNA显著高于低剂量组和对照组（P0.05），第7天高剂量组氮化应激指标显著低于低剂量组和对照组（P0.05）。光镜下第7天高剂量组小鼠肝脏肝窦充血较对照组减轻。 结论 高剂量乌司他丁可能通过抑制氮化应激保护侵袭性念珠菌感染小鼠肝脏线粒体功能，促进肝细胞线粒体生成。 　　[关键词] 侵袭性念珠菌感染；乌司他丁；线粒体；氮化应激 　　[中图分类号] R965.2 [文献标识码] A [文章编号] 1674-4721（2016）05（c）-0106-03 　　Protective effect of ulinastatin on liver mitochondrial function in mice infected by invasive Candida 　　LI Xiao-yue 　　ICU，Tangxia Hospital of Dongguan City in Guangdong Province，Dongguan 523710，China 　　[Abstract] Objective To discuss the protective effect of ulinastatin on liver mitochondrial function in mice infected by invasive Candida and its possible mechanism. Methods 60 male Kunming mice were given intraperitoneal injection of Candida albicans suspension after pretreatment by CTX and then made into models infected by invasive Candida and were randomly divided into control group （n=20），ulinastatin low-dose group （n=20），and ulinastatin high-dose group （n= 20）.Mice in the control group were given antifungal therapy，mice in the low-dose group were given antifungal drug combined with ulinastatin （1×104 U/kg），while mice in the high-dose group were given antifungal drug combined with ulinastatin （5×104 U/kg）.The survival rate，mitochondriopoiesis index，nitrosative stress index and hepatic pathology of three groups on the 7th day were observed. Results The 7th day survival rate was significantly higher in the high-dose group than that in the control group （70% vs 30%，P=0.011）.On the 7th day，the level of PGC-1α mRNA was significantly higher in the high-dose group than that in the low-dose group and the control group （P0.05），the nitrosative stress index on the 7th day was significantly lower in the high-