乙肝病毒PreS1检测在乙型肝炎诊断中作用.docVIP

乙肝病毒Pre-S1检测在乙型肝炎诊断中的作用 　　[摘要] 目的 探讨乙肝病毒(HBV)感染者血清中前S1蛋白(Pre-S1)、HBV-DNA与HBeAg的检出情况,评估Pre-S1在乙型肝炎诊断中的作用。方法 收集本院2008年1月至3月175例乙型肝炎患者的血清,采用酶联免疫吸附(ELISA)法检测HBV Pre-S1与HBV血清标志物,荧光定量PCR法检测HBV-DNA,以HBV-DNA5×10?2拷贝#8226;ml-1为阳性诊断标准。结果 不同HBV-M模式中,Pre-S1与HBV-DNA的阳性检出率比较无显著性差异(P0.05);Pre-S1与HBV-DNA的阳性符合率高于HBeAg与HBV-DNA的符合率;在HBeAg阴性患者血清中仍可不同程度地检出Pre-S1与HBV-DNA。结?论 Pre-S1可作为HBV感染、复制的一项新指标,对乙型肝炎的诊断具有重要价值。? 　　[关键词] 乙肝病毒(HBV)Pre-S1;HBV-DNA;乙肝血清标志物;病毒复制? 　　[中图分类号] R512.62 　　[文献标识码] A 　　[文章编号] 1671-7562(2009)04-0289-03 　　 　　Effect of Pre-S1 of hepatitis B virus in diagnosis of Hepatitis B 　　GAO Xia,LI Zhuo-cheng,SHE Ji-jia,CHEN Jian-xiong,LI Li-ming 　　(Department of Clinical Labolatory,the Second People?s Hospital of Shenzhen,Shenzhen 518035,China) 　　Abstract:Objective To investigate the detections of hepatitis B virus(HBV)Pre-S1,HBV-DNA and HBV pre-S1 antigen in patients? serum and to evaluate the effect of Pre-S1 antigen in diagnosis of hepatitis B.Methods In studied 175 patients, both HBV serum marker and pre-S1 antigen were detected by ELISA and HBV-DNA were detected by PCR.HBV-DNA5×10?2 copies#8226;ml-1 is set as positive for virus duplication. Results The positive ratio between Pre-S1 and HBV-DNA have no statistically significant difference in different HBV-M models(P0.05). The correlation of Pre-S1 and HBV-DNA is higher than that of HbeAg and HBV-DNA.Pre-S1 and HBV-DNA could be detected in the patients with HBeAg negative.Conclusions Pre-Sl may be used as a new and reliable serum detector for HBV infection and duplication because of its closecorrelation with ?HBV-DNA,and it can play a great role in diagnosis of hepatitis B.? 　　Key words:hepatitis B virus(HBV) pre-S1 antigen;HBV-DNA; HBV serum marker;viral replication? 　　(Modern Medical Journal,2009,37:289-291) 　　 　　近年来,对乙肝病毒(HBV)前S1蛋白(Pre-S1)的检测已引起国内外学者的关注与研究。Pre-S1是由HBV基因组S区编码的病毒衣壳蛋白,与前S2蛋白(Pre-S2)和HBsAg共同构成HBV的表面大蛋白分子,主要存在于具有传染性的Dane颗粒中,在介导HBV感染和免疫清除中具有重要作用[1]。经检测证实,HBV Pre-S1可以反映HBV在体内感染并复制的状况,是判断乙肝感染和DNA是否发