伴发抑郁对支气管哮喘大鼠P物质及皮质醇表达影响.docVIP

伴发抑郁对支气管哮喘大鼠P物质及皮质醇表达的影响 　　[摘要] 目的 探讨伴发抑郁行为后支气管哮喘大鼠体内P物质、皮?|醇含量的变化。 方法 Wistar大鼠随机分为对照组（C组）、哮喘组（A组）、哮喘伴发抑郁组（A+D组），采用卵蛋白（ovalbumin，OVA）激发法建立哮喘模型，通过慢性轻度不可预见性应激（CUMS）诱发抑郁，评价动物哮喘及抑郁表现，检测大鼠血液及肺组织P物质、皮质醇的含量。 结果 ①伴发抑郁后，哮喘大鼠肺部病变进一步加重。②与C组比较，A组、A+D组大鼠血浆及肺组织P物质含量均显著升高（P 0.05），且A+D组水平高于A组（P 0.05）。③与C组比较，A组血液及肺组织皮质醇含量明显降低（P 0.05）；而与A组比较，A+D组血液及肺组织皮质醇含量显著升高（P 0.05）。 结论 OVA激发复合CUMS可制备支气管哮喘伴发抑郁大鼠模型，该模型P物质、皮质醇含量均较单纯哮喘发生明显变化。 　　[关键词] 支气管哮喘伴发抑郁；模型；P物质；皮质醇 　　[中图分类号] R562.25 [文献标识码] A [文章编号] 1673-7210（2016）11（c）-0021-05 　　Influence of bronchial asthma with coexisted depression on levels of cortisol and substance P in rats 　　ZHANG Xiaojun1 LI Chengde1 WANG Yu1 SUN Hongwei2 MAO Shumei1 　　1.Department of Pharmacology， Weifang Medical College The Key Laboratory of Applied Pharmacology in Shandong Province， Shandong Province， Weifang 261053， China； 2.Department of Applied Psychology， Weifang Medical College， Shandong Province， Weifang 261053， China 　　[Abstract] Objective To detect the changes in levels of cortisol and substance P in rats of asthma with coexisted depression. Methods Wistar rats were randomly divided into the control group （group C）， asthma group （group A） and asthma with coexisted depression group （group A+D）. The group A and group A+D were sensitized with ovalbumin for 4 weeks. Then the group A+D was exposed to a CUMS procedure for another 4 weeks. Asthmatic manifestations and depressive-like behaviors were investigated. Levels of substance P and cortisol were measured by radioimmunoassay. Results ①The asthmatic manifestations of group A+D were worse than the group A. ②Rats in group A and group A+D had much higher levels of substance P than the rats in group C （P 0.05）； group A+D had even higher substance P levels than group A （P 0.05）. ③Levels of cortisol in group A significantly decreased if compared to the group C （P 0.05）； however， levels of cortisol in group A+D significantly increased if compared to the group A （P 0.05）. Conclusion Ovalbumin excitation